Activity of synthetic and rice putative promoters in Tobacco
| dc.contributor.advisor | Ramesh.S.Bhat | |
| dc.contributor.author | Rashmi.M.Hegde | |
| dc.date.accessioned | 2016-11-10T13:53:07Z | |
| dc.date.available | 2016-11-10T13:53:07Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | Four synthetic and two rice putative promoters were checked for inducibility in response to salicylic acid, methyl jasmonate and Cercospora nicotianae using SgfpS65T and XylanaseA reporters. T1 transgenic tobacco plants with test promoters driving SgfpS65T were infected with C. nicotianae. After 24 hr, the highest promoter activity was observed with 3 x GCC followed by 2 x S, 2 x W2 and 2 x GCC. Fold induction was highest for 2 x GCC followed by 2 x S and 2 x W2. All four synthetic promoters were stronger than CaMV 35S. Plants with test promoters driving XynA were sprayed with 5 mM salicylic acid and methyl jasmonate, and inoculated with C. nicotianae. In response to methyl jasmonate, 2 x GCC showed marginally higher promoter activity compared to 2 x W2 at 24 hr. But both the putative promoters of rice were very weak. 2 x W2 had a marginally higher fold induction compared to 2 x GCC. At no point of time, the strength of 2 x W2 and 2 x GCC promoters surpassed the level of CaMV 35S. 2 x W2 and 2 x GCC promoters showed considerably high activity in response to salicylic acid treatment, whereas rice promoters showed very week activity. 2 x W2 had marginally higher activity than 2 x GCC, which was stronger than CaMV 35S. 2 x W2 showed maximum induction compared to 2 x GCC after 12 hr of treatment. Inoculation with the pathogen could induce 2 x GCC and 2 x W2. They recorded higher promoter activity compared to rice promoters. 2 x GCC showed significantly higher promoter activity than 2 x W2 at different time intervals, but both of them were weaker than CaMV 35S. Hence 2 x GCC and 2 x W2 promoters could be employed for driving R genes. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/84868 | |
| dc.publisher | UAS, Dharwad | en_US |
| dc.research.problem | Activity of synthetic and rice putative promoters in Tobacco | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.subject | Plant Biotechnology | en_US |
| dc.theme | Activity of synthetic and rice putative promoters in Tobacco | en_US |
| dc.title | Activity of synthetic and rice putative promoters in Tobacco | en_US |
| dc.type | Thesis | en_US |