Activity of synthetic and rice putative promoters in Tobacco
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Date
2010
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Publisher
UAS, Dharwad
Abstract
Four synthetic and two rice putative promoters were checked for inducibility in
response to salicylic acid, methyl jasmonate and Cercospora nicotianae using SgfpS65T and
XylanaseA reporters. T1 transgenic tobacco plants with test promoters driving SgfpS65T were
infected with C. nicotianae. After 24 hr, the highest promoter activity was observed with 3 x
GCC followed by 2 x S, 2 x W2 and 2 x GCC. Fold induction was highest for 2 x GCC
followed by 2 x S and 2 x W2. All four synthetic promoters were stronger than CaMV 35S.
Plants with test promoters driving XynA were sprayed with 5 mM salicylic acid and
methyl jasmonate, and inoculated with C. nicotianae. In response to methyl jasmonate, 2 x
GCC showed marginally higher promoter activity compared to 2 x W2 at 24 hr. But both the
putative promoters of rice were very weak. 2 x W2 had a marginally higher fold induction
compared to 2 x GCC. At no point of time, the strength of 2 x W2 and 2 x GCC promoters
surpassed the level of CaMV 35S.
2 x W2 and 2 x GCC promoters showed considerably high activity in response to
salicylic acid treatment, whereas rice promoters showed very week activity. 2 x W2 had
marginally higher activity than 2 x GCC, which was stronger than CaMV 35S. 2 x W2
showed maximum induction compared to 2 x GCC after 12 hr of treatment. Inoculation with
the pathogen could induce 2 x GCC and 2 x W2. They recorded higher promoter activity
compared to rice promoters. 2 x GCC showed significantly higher promoter activity than 2 x
W2 at different time intervals, but both of them were weaker than CaMV 35S. Hence 2 x
GCC and 2 x W2 promoters could be employed for driving R genes.
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Keywords
Plant Biotechnology