EVALUATION OF IMMUNOLOGICAL COMPONENTS FROM THE SKIN MUCUS OF ROHU (LABEO ROHITA
| dc.contributor.advisor | K.S.RAMESH | |
| dc.contributor.author | SHIVANI, D. GOWDA | |
| dc.date.accessioned | 2023-12-18T13:58:58Z | |
| dc.date.available | 2023-12-18T13:58:58Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Teleost mucosal immunity has become the discipline of unrivalled research studies in recent years because of its diversity and defining characteristics. Skin mucus is increasingly used as a source for determining immunity-related proteins and enzymes. The aim of the present study is to understand the mucosal immunity of commercially important freshwater fish, rohu (Labeo rohita) by evaluating the immune components from its skin mucus. The study was conducted between healthy and parasite (Argulus) infested fish. Also, stress challenge was achieved with two experiments, acute crowding for 0, 2, 24 and 48 hrs; and hypoxia exposure for 0, 1 and 3 min. The IgM levels exhibited significant difference (p<0.05) between healthy and infested skin mucus as well as in case of all the experimental groups under stress challenge. SDS-PAGE, under denaturing condition gave several prominent bands of protein for healthy and infested skin mucus, wherein bands of infested sample were highly expressed comparatively; in case of stress challenge, all the experimental groups displayed protein bands. A strong antibacterial activity was observed by inhibiting the growth of tested fish pathogenic bacteria with the formation of inhibition zone. The skin mucosal lysozyme activity evidenced high significant difference (p<0.05) between healthy and infested samples. Parallel findings were observed under stress challenge for different experimental groups of acute crowding and hypoxia exposure. These results provide preliminary information for a better understanding of the role of epidermal mucus and its components in the fish innate immune system. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810203735 | |
| dc.publisher | KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR | |
| dc.sub | Aquaculture | |
| dc.theme | Aquaculture | |
| dc.these.type | M.F.Sc | |
| dc.title | EVALUATION OF IMMUNOLOGICAL COMPONENTS FROM THE SKIN MUCUS OF ROHU (LABEO ROHITA | |
| dc.type | Thesis |