MORPHO-CHEMICAL EVALUATION OF Berberis lycium Royle.

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dc.contributor.advisorSHARMA, YASHPAL
dc.contributor.authorSHARMA, VISHAL
dc.date.accessioned2023-07-14T07:07:07Z
dc.date.available2023-07-14T07:07:07Z
dc.date.issued2023-02
dc.description.abstractABSTRACT The present investigation entitled “Morpho-chemical evaluation of Berberis lycium Royle.” was carried out in the laboratory of the Department of Forest Products, College of Forestry, Dr. Y S Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.) during 2021-22. The study was conducted under three objectives namely (i) To study the effect of extraction methods and mode of drying on berberine content,(ii) To standardize HPLC method for quantification of berberine in different plant parts, (iii) To study the effect of geographical location on major bioactive compounds. For standardization of extraction techniques in B. lycium three different extraction methods viz., soxhlet extraction, reflux extraction and sonication assisted extraction were used with three different solvents. In B. lycium maximum berberine (4.05%) content was obtained in reflux extraction for sixteen hours with methanol solvent and higher total extract (16.59%) in soxhlet extraction for fourty hours with methanol as a solvent. Effect of drying methods revealed that shade drying is the best method of drying for obtaining higher total extract (13.27%) and berberine (4.05%) content. During the present studies plants of B. lycium from Himachal Pradesh at altitude ranging from 973 m to 2614 m amsl were studied. Morphological studies revealed that the maximum plant height (3.80 m) and number of branches (18.00) were recorded in plants of Sarkaghat whereas highest leaf length (4.86 cm), leaf width (1.83 cm), spine length (1.88 cm) and shoot diameter (24.73 mm) were observed in plants of Renuka Ji. Phytochemical studies revealed huge variation of berberine and other compounds in root, stem and leaf. The maximum berberine content was obtained in root (4.153%), stem (0.889%) and leaf (0.521%) of plants collected from Renuka ji whereas the maximum 4-hydroxy benzoic acid content was obtained in root (0.812%) stem (0.267%) and leaf (0.457%), chlorogenic acid content in root (0.705%), stem (3.203%), leaf (4.895%) and quercetin content in leaf were found to be maximum (0.025%) in plants collected from Churdhar. Determination of phenolic and flavonoid content revealed that the maximum phenolic content was obtained in leaf (299.63 ± 2.08 mg GAE/g of crude extract), stem (284.94 ± 1.05 mg GAE/g of crude extract) and root (209.16 ± 1.05 mg GAE/g of crude extract) and maximum flavonoid content in leaf (175.47 ± 0.72 mg RE/g of crude extract), stem (161.06 ± 1.17 mg RE/g of crude extract) and root (150.56 ± 0.66 mg RE/g of crude extract) extract was observed in plants collected from Churdhar. Simple and easy to handle HPLC method was developed and validated for quantification of berberine and other phytoconstituents in B. lycium. Method was validated as per ICH guidelines for seven parameters viz., linearity, range, accuracy, precision, LOD, LOQ and robustnessen_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810198214
dc.keywordsMorpho-chemical, evaluation, Berberis lycium Royleen_US
dc.language.isoEnglishen_US
dc.pages100+viien_US
dc.publisherUHF,NAUNIen_US
dc.subForest Productsen_US
dc.themeMorpho-chemical, evaluation, Berberis lycium Royleen_US
dc.these.typeM.Scen_US
dc.titleMORPHO-CHEMICAL EVALUATION OF Berberis lycium Royle.en_US
dc.typeThesisen_US
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