MORPHO-CHEMICAL EVALUATION OF Berberis lycium Royle.
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Date
2023-02
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UHF,NAUNI
Abstract
ABSTRACT
The present investigation entitled “Morpho-chemical evaluation of Berberis lycium Royle.” was
carried out in the laboratory of the Department of Forest Products, College of Forestry, Dr. Y S Parmar
University of Horticulture and Forestry, Nauni, Solan (H.P.) during 2021-22. The study was conducted under
three objectives namely (i) To study the effect of extraction methods and mode of drying on berberine
content,(ii) To standardize HPLC method for quantification of berberine in different plant parts, (iii) To study
the effect of geographical location on major bioactive compounds. For standardization of extraction techniques
in B. lycium three different extraction methods viz., soxhlet extraction, reflux extraction and sonication assisted
extraction were used with three different solvents. In B. lycium maximum berberine (4.05%) content was
obtained in reflux extraction for sixteen hours with methanol solvent and higher total extract (16.59%) in soxhlet
extraction for fourty hours with methanol as a solvent. Effect of drying methods revealed that shade drying is
the best method of drying for obtaining higher total extract (13.27%) and berberine (4.05%) content. During the
present studies plants of B. lycium from Himachal Pradesh at altitude ranging from 973 m to 2614 m amsl were
studied. Morphological studies revealed that the maximum plant height (3.80 m) and number of branches
(18.00) were recorded in plants of Sarkaghat whereas highest leaf length (4.86 cm), leaf width (1.83 cm), spine
length (1.88 cm) and shoot diameter (24.73 mm) were observed in plants of Renuka Ji. Phytochemical studies
revealed huge variation of berberine and other compounds in root, stem and leaf. The maximum berberine
content was obtained in root (4.153%), stem (0.889%) and leaf (0.521%) of plants collected from Renuka ji
whereas the maximum 4-hydroxy benzoic acid content was obtained in root (0.812%) stem (0.267%) and leaf
(0.457%), chlorogenic acid content in root (0.705%), stem (3.203%), leaf (4.895%) and quercetin content in leaf
were found to be maximum (0.025%) in plants collected from Churdhar. Determination of phenolic and
flavonoid content revealed that the maximum phenolic content was obtained in leaf (299.63 ± 2.08 mg GAE/g
of crude extract), stem (284.94 ± 1.05 mg GAE/g of crude extract) and root (209.16 ± 1.05 mg GAE/g of crude
extract) and maximum flavonoid content in leaf (175.47 ± 0.72 mg RE/g of crude extract), stem (161.06 ± 1.17
mg RE/g of crude extract) and root (150.56 ± 0.66 mg RE/g of crude extract) extract was observed in plants
collected from Churdhar. Simple and easy to handle HPLC method was developed and validated for
quantification of berberine and other phytoconstituents in B. lycium. Method was validated as per ICH
guidelines for seven parameters viz., linearity, range, accuracy, precision, LOD, LOQ and robustness