BIOCHEMICAL AND MOLECULAR CONFIRMATION OF RESISTANCE TO Mungbean Yellow Mosaic Virus (MYMV) IN MUNGBEAN GENOTYPES
| dc.contributor.advisor | Manjunath, B. | |
| dc.contributor.author | SHILPA, M. | |
| dc.date.accessioned | 2020-10-10T04:47:43Z | |
| dc.date.available | 2020-10-10T04:47:43Z | |
| dc.date.issued | 2020-09-30 | |
| dc.description.abstract | Among the biotic factors, Mungbean yellow mosaic virus (MYMV) is the major bottleneck for pulse producers. Fourteen mungbean genotypes procured from AVRDC, Taiwan were screened for MYMV resistance in field and laboratory conditions during Kharif 2019 and summer 2020 MYB-6, 7, 8, 9 and 12 were found to be resistant under field conditions by less PDI and AUDPC values. The confirmation of resistance was also done through estimation of biochemical enzymes like peroxidase, polyphenol oxidase, catalase, superoxide dismutase, phenols and sugars, and found comparatively higher levels in MYB-6, 7, 8, and 9 genotypes, whereas MYB-11(susceptible check) showed less activity of defense molecules. The molecular confirmation of MYMV resistance was tested using Resistant Gene Analog (RGA) in all 14 genotypes. The RGA was amplified at 90bp in resistant genotypes viz MYB-6, 7, 8 and 9. The host preference of whitefly Bemasia tabaci on mungbean genotypes was determined, no fecundity was observed on any of the mungbean lines in contrast to cotton plants. Since MYMV resistant lines MYB-6, 7, 8 and 9 showed resistance in both field and laboratory studies, hence these lines can be used as a YMV donor in breeding process and also can be release as a variety after conducting a multi location trails in MYMV hotspot conditions. | en_US |
| dc.identifier.other | Th-12608 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810153143 | |
| dc.keywords | Mungbean yellow mosaic virus,Biochemical enzymes like peroxidase, Polyphenol oxidase, catalase, superoxide dismutase, phenols and sugars. | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 101 | en_US |
| dc.publisher | UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.theme | BIOCHEMICAL AND MOLECULAR CONFIRMATION OF RESISTANCE TO Mungbean Yellow Mosaic Virus | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | BIOCHEMICAL AND MOLECULAR CONFIRMATION OF RESISTANCE TO Mungbean Yellow Mosaic Virus (MYMV) IN MUNGBEAN GENOTYPES | en_US |
| dc.type | Thesis | en_US |