BIOCHEMICAL AND MOLECULAR CONFIRMATION OF RESISTANCE TO Mungbean Yellow Mosaic Virus (MYMV) IN MUNGBEAN GENOTYPES
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Date
2020-09-30
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UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU
Abstract
Among the biotic factors, Mungbean yellow mosaic virus (MYMV) is the major
bottleneck for pulse producers. Fourteen mungbean genotypes procured from AVRDC,
Taiwan were screened for MYMV resistance in field and laboratory conditions during
Kharif 2019 and summer 2020 MYB-6, 7, 8, 9 and 12 were found to be resistant under
field conditions by less PDI and AUDPC values. The confirmation of resistance was also
done through estimation of biochemical enzymes like peroxidase, polyphenol oxidase,
catalase, superoxide dismutase, phenols and sugars, and found comparatively higher levels
in MYB-6, 7, 8, and 9 genotypes, whereas MYB-11(susceptible check) showed less
activity of defense molecules. The molecular confirmation of MYMV resistance was tested
using Resistant Gene Analog (RGA) in all 14 genotypes. The RGA was amplified at 90bp
in resistant genotypes viz MYB-6, 7, 8 and 9. The host preference of whitefly Bemasia
tabaci on mungbean genotypes was determined, no fecundity was observed on any of the
mungbean lines in contrast to cotton plants. Since MYMV resistant lines MYB-6, 7, 8 and
9 showed resistance in both field and laboratory studies, hence these lines can be used as
a YMV donor in breeding process and also can be release as a variety after conducting a
multi location trails in MYMV hotspot conditions.