STUDIES ON ACELLULAR MATRIX OF SWIM BLADDER FOR ABDOMINAL DEFECTS IN RABBITS
| dc.contributor.advisor | SREENU, MAKKENA (Major) | |
| dc.contributor.advisor | RAGHAVENDER, K B P | |
| dc.contributor.advisor | RAJU, N K B | |
| dc.contributor.author | BHANU BABU, POTINA | |
| dc.date.accessioned | 2017-05-18T08:57:18Z | |
| dc.date.available | 2017-05-18T08:57:18Z | |
| dc.date.issued | 2011-09 | |
| dc.description | THESES | en_US |
| dc.description.abstract | ABSTRACT: The present investigation deals with study of fresh, decellularized fish swim bladders and autografts for abdominal wall reconstruction in rabbits based on different parameters. Fresh swim bladders collected from fish were decellularized using 1M sodium chloride solution and 0.5% Triton X-100 under gentle agitation. This effectively removed the cellular material from the tissue and was confirmed by Haematoxylin & Eosin, Mallory’s and Van- Gieson’s staining. A total of 24 rabbits of either sex divided in to three groups were used for abdominal wall reconstruction with fresh swim bladder, processed swim bladder and autografts after creating an abdominal wall defect of 3x4cm. Diazepam as premedicant and 5% Thiopental sodium solution as intravenous agent through the marginal ear vein achieved sufficient plane of anesthesia. Parenteral administration of enrofloxacin and daily dressing of the wound with 5% povidone iodine minimized the complications of wound healing. There was an increase in the temperature, respiratory and heart rates in the immediate post operative period which returned to normal during the period of study. Total leucocyte count, glucose and ALP levels were increased in immediate post operative period where as haemoglobin, packed cell volume and total erythrocyte count were decreased in all the groups. Wound healing complications like wound dehiscence, abscessation of the wound, fistula formation and wound edges with cicatrization were evident in some animals. The fresh graft group showed variable levels of interleukins where as autograft and processed graft groups showed no appreciable levels of interleukins. The concentration of TGF β2 was high in fresh graft group followed by processed and autografts. There were no adhesions, herniation and eventration in the processed graft group where as minimal adhesions in the fresh graft and autograft groups. Neovascularization on the peritoneal side of the graft at the reconstructive site was seen by day 14 in all the groups. Histologically all the sections showed inflammatory cells, like neutrophils, monocytes and macrophages, at the host graft interface, marked fibroblastic activity towards subcutaneous layer with the formation of thin fibrous tissue and neocapillaries on the peritoneal side on 14th postoperative day in all the groups. Processed graft and autograft groups showed early mesothelialization on the peritoneal side of the graft on 7th day which prevented adhesion formation with visceral organs where as in fresh graft group mesothelialization was noticed on 14th postoperative day. Absence of foreign body giant cells suggested the biocompatibility of the both fresh and processed swim bladders with the host tissue. The processed grafts were proved to be ideal for abdominal wall reconstruction followed by fresh swim bladder and autograft. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810011439 | |
| dc.keywords | RABBITS; ABDOMINAL DEFECTS; ACELLULAR MATRIX ; SWIM BLADDER | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 129 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI – 517 502. (A.P) INDIA | en_US |
| dc.sub | Veterinary Surgery and Radiology | en_US |
| dc.subject | null | en_US |
| dc.theme | STUDIES ON ACELLULAR MATRIX OF SWIM BLADDER FOR ABDOMINAL DEFECTS IN RABBITS | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | STUDIES ON ACELLULAR MATRIX OF SWIM BLADDER FOR ABDOMINAL DEFECTS IN RABBITS | en_US |
| dc.type | Thesis | en_US |