STUDIES ON ACELLULAR MATRIX OF SWIM BLADDER FOR ABDOMINAL DEFECTS IN RABBITS
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Date
2011-09
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI – 517 502. (A.P) INDIA
Abstract
ABSTRACT:
The present investigation deals with study of fresh, decellularized fish
swim bladders and autografts for abdominal wall reconstruction in rabbits
based on different parameters. Fresh swim bladders collected from fish were
decellularized using 1M sodium chloride solution and 0.5% Triton X-100
under gentle agitation. This effectively removed the cellular material from
the tissue and was confirmed by Haematoxylin & Eosin, Mallory’s and Van-
Gieson’s staining. A total of 24 rabbits of either sex divided in to three groups were used
for abdominal wall reconstruction with fresh swim bladder, processed swim
bladder and autografts after creating an abdominal wall defect of 3x4cm.
Diazepam as premedicant and 5% Thiopental sodium solution as intravenous
agent through the marginal ear vein achieved sufficient plane of anesthesia.
Parenteral administration of enrofloxacin and daily dressing of the wound
with 5% povidone iodine minimized the complications of wound healing.
There was an increase in the temperature, respiratory and heart rates
in the immediate post operative period which returned to normal during the
period of study. Total leucocyte count, glucose and ALP levels were increased
in immediate post operative period where as haemoglobin, packed cell
volume and total erythrocyte count were decreased in all the groups. Wound
healing complications like wound dehiscence, abscessation of the wound,
fistula formation and wound edges with cicatrization were evident in some
animals. The fresh graft group showed variable levels of interleukins where as
autograft and processed graft groups showed no appreciable levels of
interleukins. The concentration of TGF β2 was high in fresh graft group
followed by processed and autografts. There were no adhesions, herniation
and eventration in the processed graft group where as minimal adhesions in
the fresh graft and autograft groups. Neovascularization on the peritoneal
side of the graft at the reconstructive site was seen by day 14 in all the
groups. Histologically all the sections showed inflammatory cells, like
neutrophils, monocytes and macrophages, at the host graft interface, marked
fibroblastic activity towards subcutaneous layer with the formation of thin
fibrous tissue and neocapillaries on the peritoneal side on 14th postoperative
day in all the groups. Processed graft and autograft groups showed early
mesothelialization on the peritoneal side of the graft on 7th day which
prevented adhesion formation with visceral organs where as in fresh graft
group mesothelialization was noticed on 14th postoperative day. Absence of
foreign body giant cells suggested the biocompatibility of the both fresh and
processed swim bladders with the host tissue. The processed grafts were
proved to be ideal for abdominal wall reconstruction followed by fresh swim
bladder and autograft.
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