Comparative Evaluation of Microbial Platform Tests with Standard Plate Count in Milk
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Date
2011-06-15
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Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar
Abstract
The quality of milk has high relevance to its physical, organoleptic, chemical and
microbiological properties as well as to its safety. Keeping the quality satisfactorily can
bring about high nutritive value of milk which is free from disease producing organisms.
In order to determine the relationship between the titratable acidity, dye reduction tests,
direct microscopic count, total bacterial count and coliform count, the present study was
undertaken by collecting the raw milk samples at various stages of milk production from
KVAFSU dairy farm and market milk; chilled milk and pasteurized milk from student
experimental dairy plant, Dairy science college, Hebbal, Bengaluru and market milk from
local market of Bengaluru.
Raw milk samples such as Aseptic, pail, can and chilled milk samples showed a
mean titratable acidity ranging between 0.14 and 0.17%, MBRT of 1.70 and 8.2 h, disc
number of 6 and 4 for RRT 10 min and 1 h. respectively Viable bacterial log count of
3.24 – 5.95 per ml and coliforms of 0.20 – 4.63 per ml. Significant difference was noticed
among parameters tested and also among the types of raw milk and market milk samples.
Positive correlation among TBC : DMC (r = 0.994) was found among the tests
conducted for the quality assessment of milk samples.
All the raw milk samples (Aseptic, pail, can, chilled milk) and market milk
samples were subjected to shelf life study by storing at room temperature (260C) and
refrigeration temperature (60C). Aseptic milk, pail milk, can milk, chilled milk, market
milk samples (B1 to B4) showed keeping quality of 18, 11, 9, 8, 26 ½ h respectively at
ambient temperature and positive correlation of r = 0.932 was noticed between TBC and
DMC at room temperature among milk samples at refrigeration temperature (40C),
Aseptic milk, pail milk, can milk, chilled milk and market milk samples showed keeping
quality of 36, 25, 20, 16 and 30 ½ h respectively. Positive correlation of r = 0.573 was
noticed between TBC and MBRT at refrigeration temperature among the milk samples.
Description
PG, Thesis
Keywords
Comparative Evaluation of Microbial Platform Tests with Standard Plate Count in Milk