Serodiagnosis and Molecular characterization of Bhendi Yellow vein mosaic virus
| dc.contributor.advisor | M.S.Patil | |
| dc.contributor.author | T.L.Prakasha | |
| dc.date.accessioned | 2016-11-02T17:49:04Z | |
| dc.date.available | 2016-11-02T17:49:04Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | Bhendi (Abelmoschous esculentus (L.) Moench is one of the important vegetable crops grown in Karnataka. Bhendi crop suffers from several biotic stresses among the important diseases of this crop Bhendi yellow vein mosaic disease is one of the most severe diseases leading to heavy losses of the crop. Roving survey conducted during kharif 2008 and summer season 2009 in four districts of Karnataka indicated more severe disease incidence during summer season with disease incidence varying from 15.2 to 48.9 per cent. Maximum incidence of 48.9 per cent was noticed in UAS, Dharwad campus. During kharif, incidence varied from 0 to 19.5 per cent. The bioassay and RAPD-PCR analysis of whiteflies revealed the presence of Bbiotype in Dharwad on both cotton and bhendi which is a potential vector for the transmission of BYVMV. Gene encoding the coat protein of BYVMV had 771 bp which codes for 256 amino acids. It shared highest nucleotide (99.35%) and amino acid (99.65%) sequence identity with BYVMV Madhurai Variant 3 (AJ278861) and Barrackpur isolate (EF417918). Antiserum was produced in rabbits using partially purified virus preparation and DAS-ELISA was standardized and successfully used to detect BYVMV in infected leaves. Out of 16 field samples tested in DAS-ELISA, only two samples viz., bhendi and Ageratum conizoides showed positive reaction Sugars content was increased due to infection of BYVMV and the sugar content viz., Reducing sugars, non-reducing sugars and total sugars was found to be more in the susceptible variety (Pusa Sawani) than resistant variety (Arka Anamika). Phenol content and enzymatic activities (PAL, Chitinase and Peroxidase) were increased in BYVMV infected leaves rather than healthy leaves and their activity were low in susceptible variety when compared with the resistant variety. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/83510 | |
| dc.publisher | UAS, Dharwad | en_US |
| dc.research.problem | Serodiagnosis and Molecular characterization of Bhendi Yellow vein mosaic virus | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.theme | Serodiagnosis and Molecular characterization of Bhendi Yellow vein mosaic virus | en_US |
| dc.these.type | M.Sc | |
| dc.title | Serodiagnosis and Molecular characterization of Bhendi Yellow vein mosaic virus | en_US |
| dc.type | Thesis | en_US |