Serodiagnosis and Molecular characterization of Bhendi Yellow vein mosaic virus
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Date
2009
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UAS, Dharwad
Abstract
Bhendi (Abelmoschous esculentus (L.) Moench is one of the important vegetable
crops grown in Karnataka. Bhendi crop suffers from several biotic stresses among the
important diseases of this crop Bhendi yellow vein mosaic disease is one of the most severe
diseases leading to heavy losses of the crop. Roving survey conducted during kharif 2008 and
summer season 2009 in four districts of Karnataka indicated more severe disease incidence
during summer season with disease incidence varying from 15.2 to 48.9 per cent. Maximum
incidence of 48.9 per cent was noticed in UAS, Dharwad campus. During kharif, incidence
varied from 0 to 19.5 per cent.
The bioassay and RAPD-PCR analysis of whiteflies revealed the presence of Bbiotype
in Dharwad on both cotton and bhendi which is a potential vector for the
transmission of BYVMV. Gene encoding the coat protein of BYVMV had 771 bp which
codes for 256 amino acids. It shared highest nucleotide (99.35%) and amino acid (99.65%)
sequence identity with BYVMV Madhurai Variant 3 (AJ278861) and Barrackpur isolate
(EF417918).
Antiserum was produced in rabbits using partially purified virus preparation and
DAS-ELISA was standardized and successfully used to detect BYVMV in infected leaves.
Out of 16 field samples tested in DAS-ELISA, only two samples viz., bhendi and Ageratum
conizoides showed positive reaction
Sugars content was increased due to infection of BYVMV and the sugar content viz.,
Reducing sugars, non-reducing sugars and total sugars was found to be more in the
susceptible variety (Pusa Sawani) than resistant variety (Arka Anamika). Phenol content and
enzymatic activities (PAL, Chitinase and Peroxidase) were increased in BYVMV infected
leaves rather than healthy leaves and their activity were low in susceptible variety when
compared with the resistant variety.