ISOLATION AND MOLECULAR CHARACTERIZATION OF Pseudomonas SPECIES AND ITS EFFECT ON PLANT GROWTH
| dc.contributor.advisor | HARINIKUMAR, K. M. | |
| dc.contributor.author | SREENIVASAMURTHY, G. T. | |
| dc.date.accessioned | 2020-10-06T04:45:56Z | |
| dc.date.available | 2020-10-06T04:45:56Z | |
| dc.date.issued | 2019-08-27 | |
| dc.description.abstract | Fluorescent Pseudomonads belong to plant Growth Promoting Rhizobacteria (PGPR), the important group of bacteria that play a major role in the plant growth promotion, induced systemic resistance, biological control of pathogens etc. Many strains of Pseudomonas fluorescens are known to enhance plant growth promotion and reduce severity of various diseases. This investigation was carried out to study the molecular and physiological characterization of Pseudomonas species isolated from different locations of Tumakuru and Bengaluru district and its effects on the root growth of ground nut and finger millet . Pseudomonas strains from different locations were isolated, identified and confirmed as Pseudomonas fluorescens using standard synaptic keys. These included microscopic observation, biochemical and physiological characters like oxidase test, catalase test, casein hydrolysis test, starch hydrolysis and growth studies at 40C and 410C. Isolates were identified and characterized as Pseudomonas fluorescens. Molecular diversity of these isolates were characterized by 16S rDNA universal primers analysis. out of twelve isolates best performing six isolates enhanced root growth were sent for sequencing and the sequence obtained were compared against sequences on the GenBank at the NCBI website. Analysis of the sequences by BLAST tool on the NCBI website indicated that isolate PS-GN, PS-T, PS-FM matched 97% with Pseudomonas fluorescens. Simultaneously plant growth response studies using groundnut and finger millet as host were conducted. In plants inoculated with P. fluorescens isolates, the root length, fresh and dry weight of roots, remained higher than the uninoculated plants. In the experiment conducted, isolates of rhizospheric soil selected from ground nut (PS-GN) tomato (PST) and fingermillet (PS-FM) performed well compared to other isolates with respect to all the parameters mentioned above. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810152477 | |
| dc.keywords | Isolation and Molecular, Fluorescent Pseudomonads ,Plant Growth ,Promoting Rhizobacteria . | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 103 | en_US |
| dc.publisher | UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.theme | ISOLATION AND MOLECULAR CHARACTERIZATION | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | ISOLATION AND MOLECULAR CHARACTERIZATION OF Pseudomonas SPECIES AND ITS EFFECT ON PLANT GROWTH | en_US |
| dc.type | Thesis | en_US |