ISOLATION AND MOLECULAR CHARACTERIZATION OF Pseudomonas SPECIES AND ITS EFFECT ON PLANT GROWTH
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Date
2019-08-27
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UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU
Abstract
Fluorescent Pseudomonads belong to plant Growth Promoting Rhizobacteria
(PGPR), the important group of bacteria that play a major role in the plant growth
promotion, induced systemic resistance, biological control of pathogens etc. Many strains
of Pseudomonas fluorescens are known to enhance plant growth promotion and reduce
severity of various diseases. This investigation was carried out to study the molecular and
physiological characterization of Pseudomonas species isolated from different locations
of Tumakuru and Bengaluru district and its effects on the root growth of ground nut and
finger millet . Pseudomonas strains from different locations were isolated, identified and
confirmed as Pseudomonas fluorescens using standard synaptic keys. These included
microscopic observation, biochemical and physiological characters like oxidase test,
catalase test, casein hydrolysis test, starch hydrolysis and growth studies at 40C and 410C.
Isolates were identified and characterized as Pseudomonas fluorescens. Molecular
diversity of these isolates were characterized by 16S rDNA universal primers analysis.
out of twelve isolates best performing six isolates enhanced root growth were sent for
sequencing and the sequence obtained were compared against sequences on the GenBank
at the NCBI website. Analysis of the sequences by BLAST tool on the NCBI website
indicated that isolate PS-GN, PS-T, PS-FM matched 97% with Pseudomonas fluorescens.
Simultaneously plant growth response studies using groundnut and finger millet as host
were conducted. In plants inoculated with P. fluorescens isolates, the root length, fresh
and dry weight of roots, remained higher than the uninoculated plants. In the experiment
conducted, isolates of rhizospheric soil selected from ground nut (PS-GN) tomato (PST) and fingermillet (PS-FM) performed well compared to other isolates with respect to
all the parameters mentioned above.