GENETIC IDENTIFICATION OF APPLE GENOTYPES BY USING MOLECULAR MARKERS
| dc.contributor.advisor | MODGIL, MANJU | |
| dc.contributor.author | PATHANIA, PARDEED | |
| dc.date.accessioned | 2017-01-20T14:37:02Z | |
| dc.date.available | 2017-01-20T14:37:02Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | ABSTRACT Random amplified polymorphic DNA (RAPDs) and simple sequence repeats (SSR) were used to test the genetic variability and relatedness among 20 cultivars of apple. 28 RAPD and 5 SSR primers were used to amplify the DNAs of all 20 cultivars. RAPD generated 107 polymorphic bands with 65.6% polymorphism while SSR generated 15 polymorphic alleles with 71.4% polymorphism. The average PIC value was 0.70 for RAPD and 0.64 for SSR and the PIC value was the highest in OPA-16(RAPD) and IISRS-3; IISRS-18(SSR) primers. RAPD and SSR revealed different genetic similarities among 20 apple cultivars. The highest similarity was detected between ‘Fuji’ and ‘Fuji Rekaki’ (0.859) followed by ‘Thanedar early flowering’ and ‘Fuji’ by RAPD while between ‘Galaxy’ and ‘Jonadel’ (1.000) followed by ‘Neomi’ with ‘Arlet’, ‘Fuji’ and ‘Royal Gala’; ‘Fuji Rekaki’ with ‘Thanedar early flowering’ and ‘Reinette-Du-Canada’ by SSR. The dendrogram generated from RAPD data grouped ‘Fuji’ and ‘Fuji Rekaki’ in one cluster and ‘Jonadel’ and ‘Jonagold’ in other cluster, while dendrogram derived from SSR data grouped ‘Jonadel’ and ‘Jonagold’ in one cluster and ‘Arlet’ and ‘Royal Gala’ in other cluster, which is generally in accordance with the recorded pedigree information | en_US |
| dc.identifier.other | 47353 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/97415 | |
| dc.language.iso | en | en_US |
| dc.sub | Biotechnology | en_US |
| dc.these.type | M.Sc | |
| dc.title | GENETIC IDENTIFICATION OF APPLE GENOTYPES BY USING MOLECULAR MARKERS | en_US |
| dc.type | Thesis | en_US |