GENETIC IDENTIFICATION OF APPLE GENOTYPES BY USING MOLECULAR MARKERS
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Date
2010
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Abstract
ABSTRACT
Random amplified polymorphic DNA (RAPDs) and simple sequence repeats
(SSR) were used to test the genetic variability and relatedness among 20 cultivars of
apple. 28 RAPD and 5 SSR primers were used to amplify the DNAs of all 20
cultivars. RAPD generated 107 polymorphic bands with 65.6% polymorphism while
SSR generated 15 polymorphic alleles with 71.4% polymorphism. The average PIC
value was 0.70 for RAPD and 0.64 for SSR and the PIC value was the highest in
OPA-16(RAPD) and IISRS-3; IISRS-18(SSR) primers. RAPD and SSR revealed
different genetic similarities among 20 apple cultivars. The highest similarity was
detected between ‘Fuji’ and ‘Fuji Rekaki’ (0.859) followed by ‘Thanedar early
flowering’ and ‘Fuji’ by RAPD while between ‘Galaxy’ and ‘Jonadel’ (1.000)
followed by ‘Neomi’ with ‘Arlet’, ‘Fuji’ and ‘Royal Gala’; ‘Fuji Rekaki’ with
‘Thanedar early flowering’ and ‘Reinette-Du-Canada’ by SSR. The dendrogram
generated from RAPD data grouped ‘Fuji’ and ‘Fuji Rekaki’ in one cluster and
‘Jonadel’ and ‘Jonagold’ in other cluster, while dendrogram derived from SSR data
grouped ‘Jonadel’ and ‘Jonagold’ in one cluster and ‘Arlet’ and ‘Royal Gala’ in
other cluster, which is generally in accordance with the recorded pedigree
information