“CHARACTERIZATION OF PHYTOPHTHORA BLIGHT RESISTANT AND SUSCEPTIBLE SESAME (sesamum indicum L.) GENOTYPES USING MOLECULAR MARKERS”

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dc.contributor.advisorDr. S. V. Patel
dc.contributor.authorMr. Karad Kiritkumar Dhanjibhai
dc.date.accessioned2017-07-14T09:00:34Z
dc.date.available2017-07-14T09:00:34Z
dc.date.issued2015-06
dc.description.abstractThe present investigation on “CHARACTERIZATION OF PHYTOPHTHORA BLIGHT RESISTANT AND SUSCEPTIBLE SESAME (sesamum indicum L.) GENOTYPES USING MOLECULAR MARKERS” was conducted at Department of Biotechnology, Junagadh Agricultural University, Junagadh with objectives, to screen sesame genotypes for Phytophthora blight resistance and susceptible based on molecular markers viz. Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Simple Sequence Repeats (SSR). Total 20 RAPD primers generated 299 bands in which 279 were polymorphic with an average of 13.95 bands and 94.2% polymorphism per primer. The RAPD primers augmented fragment size was ranged from 132 bp-3600 bp. The 12 ISSR primers engendered 133 bands in which 126 bands were polymorphic that was equal to 95.60% polymorphism with an average of 10.5 bands per primer. The amplified fragments were in range of 130-2538 bp. Total 19 SSR primers generated the 117 fragments in which 89 bands were polymorphic with 70.25% polymorphism and average of 4.68 bands per primer. The similarity coefficient of clusters analysis was ranged from 31 to 79% for RAPD, 32 to 75% for ISSR and 53 to 79% for SSR. The pooled study of RAPD, ISSR and SSR generated clustering pattern which seem to be similar as ISSR clustering pattern. The 10 RAPD primers (B8, OPA-01, OPA-02, OPA-03, OPA-11, OPA-19, OPC-13, OPD-12, OPE-08 and OPM-13), 6 ISSR primers (ISSR 1, ISSR 6, ISSR 10, ISSR 16, ISSR 17 and ISSR 18) and 7 SSR primers (AY838909, AY838916, SI-ssr11, SI-ssr29, SI-ssr30, SEM-12-27 and SEM-12-147) produced genotype specific bands and discriminated six, eight and nine genotypes across the 16 sesame genotypes, respectively. Among all molecular marker studied, ISSR markers seem to be more informative than RAPD and SSR to distinguish Phytophthora blight resistant and susceptible sesame genotypes. Key words: Sesame, Phytophthora blight, RAPD, ISSR, SSR, sesamum indicum L.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810025253
dc.language.isoenen_US
dc.pages130en_US
dc.publisherjau,junagdhen_US
dc.subAgricultural Biotechnologyen_US
dc.subjectBIOTECHNOLOGYen_US
dc.theme“CHARACTERIZATION OF PHYTOPHTHORA BLIGHT RESISTANT AND SUSCEPTIBLE SESAME (sesamum indicum L.) GENOTYPES USING MOLECULAR MARKERS”en_US
dc.these.typeM.Scen_US
dc.title“CHARACTERIZATION OF PHYTOPHTHORA BLIGHT RESISTANT AND SUSCEPTIBLE SESAME (sesamum indicum L.) GENOTYPES USING MOLECULAR MARKERS”en_US
dc.typeThesisen_US
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