PREVALENCE AND CHARACTERIZATION OF LISTERIA SPECIES AT DIFFERENT ECO-EPIDEMIOLOGICAL UNITS OF ANIMAL INTERFACE
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
The current study was undertaken with the objective of studying Listeria species
from different eco-epidemiological units of animal interface in the rural and peri-urban
areas of Shivamogga district. A total of 310 samples were collected, analysed,
characterized by biochemical, sugar fermentation and molecular methods. The overall
prevalence of Listeria species was found to be 2.5%. Epidemiological unit wise, the
prevalence of Listeria species was found to highest in the (Abbalgere) 4.4%, (Massur)
4% followed by (Bhadravathi) 3.63%, (Anupinakatte) 3.63% and (L.F.C, VCS) 1.42%.
Culturally, identified Listeria species were subjected to PCR targeting of 16SrRNA,
Lmo1030, namA, scrA and Oxidoreductasi genes. Further, the isolates were subjected
to PCR targeting the virulence associated genes viz., plcA, hlyA, iap and prfA. Out of
310 samples eight isolates were identified as L.monocytogenes (0.6%), L. ivanovii
(0.6%), L. welshimeri (0.6%) and L. grayi (0.6%). Genetic diversity of the isolates
were performed by ERIC PCR where six ERIC types/cluster (C1to C6) were found with
the Shannon weiver index of 0.752 and Simpson index of 0.928. Antibiogram showed
that the eight Listeria species where resistant to bacitracin (62.5%) and cefotaxamie
(62.5%), whereas susceptible to ciprofloxacin (62.5%), chloramphenicol (50%) and
gentamicin (50%). The MAR index of L. monocytogenes (F113 and M9) was found to
be 0.25 and 0.18, L. ivanovii (F82 and VS6) was 0.0625 and 0.125, L. welshimeri (
PF4 and F118) was 0.125 and 0.3125 and L.grayi (S3 and SD1) was 0.0625 and 0.125,
respectively. L. monocytogenes and L. ivanovii showed weak biofilm ability at 24hrs
were as, L.welshimeri and L.grayi were non biofilm formers. At 48 hrs, L.
monocytogenes were moderate biofilm formers and at 72 hrs, L.monocytogenes were
strong biofilm formers, L.ivanovii moderate biofilm formers, L.welshimeri and L.grayi
were weak biofilm formers
KEY WORDS: 16SrRNA, ERIC-PCR, AST, Biofilm