STUDY ON THE EFFECT OF ANTIMICROBIAL RESISTANT PATHOGENS OF BOVINE MASTITIS AND LACTIC ACID BACTERIA IN DAHI
| dc.contributor.advisor | PRABHA. R | |
| dc.contributor.author | GANESH | |
| dc.date.accessioned | 2023-12-29T10:58:55Z | |
| dc.date.available | 2023-12-29T10:58:55Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | In the present study 45 isolates of bovine mastitis were obtained from Veterinary Microbiology Department, KVAFSU, Bengaluru. The purified 40 Gram-positive isolates and 5 of Gram negative, were identified as Streptococcus uberis (10), Streptococcus agalactiae (12); one each of Staphylococcus delphini, Staphylococcus carnosus and Staphylococcus gallanarium while 6 each of Staphylococcus equorum & Staphylococcus epidermis and 3 Staphylococcus aureus. Gram negative 5 isolates were identified as E. coli (3), Salmonella enterica (1) and Alcaligenes sp. (1). All the isolates when subjected to antibiotic susceptibility test (18 antibiotics), Streptococcus agalactiae M36, Staphylococcus aureus M35, E. coli M33, Salmonella enterica M27 showed highest resistance for 16, 15, 7, 5 antibiotics, respectively. The highest antibiotic resistant isolates were PCR analysed for their speciation. Lactococcus lactic ssp. lactis 1, Lactococcus lactic ssp. lactis 2, Lactococcus lactic ssp. cremoris were used in dahi preparation alone or in combination along with selected mastitic causing bacteria having public health significance and highly resistant to antibiotics. The prepared dahi had viable count of around 8.6 both in individual and in mixed lactic cultures with lactic acid content of 0.77%. Lactic cultures exhibited resistance to ampicillin, azithromycin, cefoperazone, ceftriaxone, nalidixic acid, penicillin, rifampicin and vancomycin. Mastitic bacterial isolates, showed resistant to all the antibiotics, while the Streptococcus agalactiae and E. coli showed susceptibility to streptomycin. In dahi prepared using mixed lactic culture, there may be possibility of transfer of resistant factor of ceftriaxone to Salmonella enterica ssp. enterica M27 and both ceftriaxone and ofloxacin resistance to Staphylococcus aureus M35 from lactic cultures which were resistant in dahi while pathogens in broth were susceptible. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810204933 | |
| dc.language.iso | English | |
| dc.pages | 179 | |
| dc.publisher | KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR-585 401 | |
| dc.sub | Dairy Microbiology | |
| dc.theme | Dairy Microbiology | |
| dc.these.type | M.Tech. | |
| dc.title | STUDY ON THE EFFECT OF ANTIMICROBIAL RESISTANT PATHOGENS OF BOVINE MASTITIS AND LACTIC ACID BACTERIA IN DAHI | |
| dc.type | Thesis |