Identification and characterization of “Landmark Protein” from Rhizobium isolates of Cicer arietinum from acidic soil of Jharkhand
| dc.contributor.advisor | Dubey, Himanshu | |
| dc.contributor.author | Kumari, Neha | |
| dc.date.accessioned | 2019-02-21T11:00:47Z | |
| dc.date.available | 2019-02-21T11:00:47Z | |
| dc.date.issued | 2018 | |
| dc.description | Identification and characterization of “Landmark Protein” from Rhizobium isolates of Cicer arietinum from acidic soil of Jharkhand | en_US |
| dc.description.abstract | The soil pH is a measure of the acidity or alkalinity in soils. Soil pH is considered as an important factor of soils as it controls many chemical processes that take place in plants and microorganisms that live in soil. Nitrogen is the most common deficient nutrient in many soils around the world and supply of nitrogen through fertilizers has severe environmental concerns. Nitrogen cannot be absorbed by plants directly from atmosphere, but only in the form of salts of nitrogen. Rhizobia are soil bacteria and are gram negative, motile, non sporulating rods that fix nitrogen i.e. convert atmospheric nitrogen into ammonia (ammonia acts as a natural fertilizer for the plant) after becoming established inside root nodules of legumes (Fabaceae). The plant in turn provides the bacteria with organic compounds made by photosynthesis. The symbiotic relationship implies a signal exchange between both partners. There are various environmental factors like soil pH, temperature etc. which affects the survival of Rhizobia as well as the nodulation process and thus the N2 fixation. Chickpea (Cicer arietinum L.) is one of the important pulses cultivated in India during winter season. It is a good source of carbohydrates and proteins which together constitute about 80% of the total dry seed mass and the protein quality is considered to be better than other pulses. It has significant amounts of all the essential amino acids except sulfur containing types. It is cholesterol free and is a good source of dietary fiber, vitamins and minerals. Proteomics is a large scale study of proteins. Two-dimensional electrophoresis (2-D electrophoresis) is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. This technique sorts proteins according to two independent properties in two discrete steps: the first-dimension step, isoelectric focusing (IEF), separates proteins according to their Isoelectric Points (pI); the second-dimension step, SDSpolyacrylamide gel electrophoresis (SDS-PAGE), separates proteins according to their molecular weights. Each spot on the resulting two-dimensional array corresponds to a single protein species in the sample. Thousands of different proteins can thus be separated, and information such as the protein pI, the apparent molecular weight, and the amount of each protein is obtained. The aim of my work is to identify and characterize of “Landmark Protein” from Rhizobium isolates of Chickpea (Cicer arietinum L.) collected from various soil regimes of Jharkhand. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810096373 | |
| dc.keywords | Identification and characterization of “Landmark Protein” from Rhizobium isolates of Cicer arietinum from acidic soil of Jharkhand | en_US |
| dc.language.iso | en_US | en_US |
| dc.pages | 63 | en_US |
| dc.publisher | Birsa Agricultural University, Ranchi, Jharkhand-6 | en_US |
| dc.sub | Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | Identification and characterization of “Landmark Protein” from Rhizobium isolates of Cicer arietinum from acidic soil of Jharkhand | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Identification and characterization of “Landmark Protein” from Rhizobium isolates of Cicer arietinum from acidic soil of Jharkhand | en_US |
| dc.type | Thesis | en_US |