Characterization of Rhizobium isolates of Soybean collected from the acidic soils of the state of Jharkhand
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Date
2019
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Birsa Agricultural University, Ranchi
Abstract
Out of several gases present in the atmosphere nitrogen share the major portion (about 71%) and
is found in the di-nitrogen (an inert) form. It is the component of many bio-molecules required
for the growth and development of all organisms. Most of the eukaryotes are incapable of
utilizing nitrogen directly from the environment; only a certain group of prokaryotes are
genetically feasible to fix the atmospheric nitrogen into the biologically useful form like
ammonia which is further utilized by eukaryotes. Rhizobium, a gram negative bacteria associates
symbiotically with legume crop and are genetically feasible in reducing (fixing) atmospheric
nitrogen for leguminous crop. Legumes in turn provide shelter and energy to them. The
specificity of Rhizobia to inoculate legume falls either in broad range host specificity to narrow
range host specificity. Several abiotic stresses adversely influence the activity of Rhizobium. Soil
pH is one of the stresses which hamper the symbiotic association between the two. As per the
reports soil pH in the range of 6.5-7.0 are considered best in the case of leguminous crop for the
optimal activity of the bacteria. Soil pH below or above this range minimizes the Biological
Nitrogen Fixation (BNF) through Rhizobia. My work is to characterize the Rhizobium isolates of
soybean collected from acidic soils of the state of Jharkhand.
Through studying the proteome of Rhizobium in acidic soil condition, the response of the isolates
towards acidity of soil is being analyzed. At the molecular level, we find that the two
dimensional gel analysis reveals a host of proteins which are found to be up-regulated or down
regulated in response to different pH conditions. We hypothesize that the protein changes
observed on two-dimensional electrophoresis in response to different pH of acidic soil reflected
the molecular adaptation mechanism taking place in progress in soybean to combat and recover
in response to abiotic stress such as acidic soil.
Description
Characterization of Rhizobium isolates of Soybean
collected from the acidic soils of the state of
Jharkhand