STUDIES ON SOYBEAN MOSAIC VIRUS DISEASE
| dc.contributor.advisor | PRAMEELA, H. A. | |
| dc.contributor.author | MAHIPALREDDY | |
| dc.date.accessioned | 2018-07-25T03:44:04Z | |
| dc.date.available | 2018-07-25T03:44:04Z | |
| dc.date.issued | 2017-12-27 | |
| dc.description.abstract | Soybean mosaic virus (SMV) disease caused by a potyvirus is a major constraint in many soybean producing areas. Soybean cv. JS-335 showed 84.00 to 96.00 per cent virus transmission upon mechanical inoculation. The virus was found to be highly stable at pH 6.0. Addition of 0.5 per cent sodium sulphite to phosphate buffer resulted in maximum transmission of virus. Seed transmission of SMV varied from five to ten per cent. Aphid, Myzus persicae Sulz. transmitted the virus from 45.00 to 60.00 per cent. The optimum preacquisition starvation period was one hour, at which 40.00 per cent virus transmission observed. A single viruliferous aphid was able to transmit the virus. The optimum acquisition access period was one minute that resulted in 40.00 per cent transmission. The optimum inoculation access period was 30 seconds at which 50 per cent transmission occurred. In the host range studies soybean, field bean, french bean, cowpea, cucumber, Datura took SMV infection and exhibited mosaic symptoms, whereas Chenopodium exhibited chlorotic local lesions. The virus was detected by DAC-ELISA using potyvirus specific antisera. The RT-PCR product of 500 bp corresponding to coat protein (CP) genes of the virus was amplified from total RNA obtained from SMV infected leaf sample. Of the 500 soybean genotypes screened against SMV under field conditions, none of the genotypes were found immune to SMV disease however, 45 genotypes were resistant and 341 were moderately resistant to soybean mosaic virus disease. | en_US |
| dc.identifier.other | Th-11891 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810061814 | |
| dc.language.iso | en | en_US |
| dc.pages | 137 | en_US |
| dc.publisher | UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.subject | null | en_US |
| dc.theme | STUDIES ON SOYBEAN MOSAIC VIRUS DISEASE | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | STUDIES ON SOYBEAN MOSAIC VIRUS DISEASE | en_US |
| dc.type | Thesis | en_US |