STUDIES ON SOYBEAN MOSAIC VIRUS DISEASE
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Date
2017-12-27
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Soybean mosaic virus (SMV) disease caused by a potyvirus is a major constraint in
many soybean producing areas. Soybean cv. JS-335 showed 84.00 to 96.00 per cent virus
transmission upon mechanical inoculation. The virus was found to be highly stable at pH
6.0. Addition of 0.5 per cent sodium sulphite to phosphate buffer resulted in maximum
transmission of virus. Seed transmission of SMV varied from five to ten per cent. Aphid,
Myzus persicae Sulz. transmitted the virus from 45.00 to 60.00 per cent. The optimum preacquisition
starvation period was one hour, at which 40.00 per cent virus transmission
observed. A single viruliferous aphid was able to transmit the virus. The optimum
acquisition access period was one minute that resulted in 40.00 per cent transmission. The
optimum inoculation access period was 30 seconds at which 50 per cent transmission
occurred. In the host range studies soybean, field bean, french bean, cowpea, cucumber,
Datura took SMV infection and exhibited mosaic symptoms, whereas Chenopodium
exhibited chlorotic local lesions. The virus was detected by DAC-ELISA using potyvirus
specific antisera. The RT-PCR product of 500 bp corresponding to coat protein (CP) genes
of the virus was amplified from total RNA obtained from SMV infected leaf sample. Of
the 500 soybean genotypes screened against SMV under field conditions, none of the
genotypes were found immune to SMV disease however, 45 genotypes were resistant and
341 were moderately resistant to soybean mosaic virus disease.
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