Detection of Mycoplasma gallinaceum by PCR amplification of the 16S rRNA gene from respiratory disease in village chickens

Simple item page
dc.contributor.authorPonnuswamy, P
dc.contributor.authorLurthu Reetha, T
dc.contributor.authorRonald, BSM
dc.contributor.authorPuvarajan, B
dc.contributor.authorManickam, R
dc.contributor.authorTANUVAS
dc.date.accessioned2019-12-27T10:18:44Z
dc.date.available2019-12-27T10:18:44Z
dc.date.issued2018
dc.descriptionTNV_IJAR_2018_1-4en_US
dc.description.abstractIn the present study, Mycoplasma gallinaceum was detected by PCR amplification of 16S rRNA gene from chronic respiratory disease in village chickens of Cauvery delta region of Tamil Nadu. Necropsy was performed to find out the etiological agent in desi birds mortality. At necropsy, airsacculitis with caseous exudate were found in the thoracic and abdominal cavity. Caseous material from airsacs was collected aseptically from dead birds for detection of Mycoplasma species. DNA was extracted from caseous material by using tissue DNA extraction kit. PCR was carried out using primers to amplify 16S rRNA gene belonging to Mycoplasma species. The amplified product yielded approximately 700-bp length (703 to 713 bp) of the 16S rRNA gene specific for Mycoplasma species. Further, it was subjected to sequence analysis and confirmed as Mycoplasma gallinaceum by NCBI blast analysis. In the present communication, detection of M. gallinaceum by PCR amplification of 16S rRNA gene provides a powerful tool for rapid diagnosis.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810138903
dc.keywordsM. Gallinaceum, 16S rRNA sequence analysis, Native chicken.en_US
dc.language.isoenen_US
dc.pages1-4en_US
dc.subjectVeterinary Scienceen_US
dc.titleDetection of Mycoplasma gallinaceum by PCR amplification of the 16S rRNA gene from respiratory disease in village chickensen_US
dc.title.alternativeIndian Journal of Animal Researchen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
TNV_IJAR_2018_1-4.pdf
Size:
150.25 KB
Format:
Adobe Portable Document Format
Description:
TNV_IJAR_2018_1-4
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Articles (1.Journal)