Detection of Mycoplasma gallinaceum by PCR amplification of the 16S rRNA gene from respiratory disease in village chickens
Loading...
Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
In the present study, Mycoplasma gallinaceum was detected by PCR amplification of 16S rRNA gene from chronic respiratory
disease in village chickens of Cauvery delta region of Tamil Nadu. Necropsy was performed to find out the etiological
agent in desi birds mortality. At necropsy, airsacculitis with caseous exudate were found in the thoracic and abdominal
cavity. Caseous material from airsacs was collected aseptically from dead birds for detection of Mycoplasma species. DNA
was extracted from caseous material by using tissue DNA extraction kit. PCR was carried out using primers to amplify 16S
rRNA gene belonging to Mycoplasma species. The amplified product yielded approximately 700-bp length (703 to 713
bp) of the 16S rRNA gene specific for Mycoplasma species. Further, it was subjected to sequence analysis and confirmed
as Mycoplasma gallinaceum by NCBI blast analysis. In the present communication, detection of M. gallinaceum by PCR
amplification of 16S rRNA gene provides a powerful tool for rapid diagnosis.
Description
TNV_IJAR_2018_1-4
Keywords
Veterinary Science