STUDIES ON FELINE PANLEUKOPENIA IN AND AROUND BENGALURU
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
The present study was undertaken to investigate the occurrence and haematobiochemical
changes of FPL in and around Bengaluru. The study also aimed at comparing
immunochromatography with PCR for detection of FPLV from faecal samples of FPL
suspected cats.
PCR assay detected DNA of feline panleukopenia virus in 73.08 per cent faecal
samples of suspected cats. FPL was shown to be more common among cats under six
months old, males and nondescript breed of cats based on epidemiological data. Outdoor
cats and non-dewormed cats were more likely to be infected. The most common clinical
findings were noticed includes vomiting, diarrhoea, pyrexia/low temperature, dehydration,
dullness/weakness, and anorexia. Leukopenia, thrombocytopenia, anaemia, increased AST
and creatinine, hypoproteinemia and hypoalbuminemia were the haemato-biochemical
findings of cats affected with FPL.
Immunochromatographic strips (IC) and polymerase chain reaction (PCR) were
used to detect FPLV from faecal samples of FPL suspected cats. PCR showed positive
results in 73.08 per cent samples examined. The VP2 gene of FPV was targeted with PCR,
which yielded 698 bp amplicons that were sequenced. BLAST analysis of the sequence
revealed 99.85 homology to FPLV sequences available in the NCBI GenBank. The
sensitivity and specificity of IC strip test was determined by comparing its results with
PCR. Based on statistical analysis, the specificity of IC was good (100 %), whereas the
sensitivity was poor (47.6 %) when compared to PCR. Thus, it is concluded that PCR is
more sensitive than the IC in detecting FPLV in faeces.
Key Words: Feline panleukopenia (FPL), Immunochromatography (IC), PCR