Isolation and characterization of Toxoplasma gondii from small ruminants (sheep and goats) in Chennai City, South India

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dc.contributor.authorSatbige, Ajay Suryakant
dc.contributor.authorSreekumar, C.
dc.contributor.authorRajendran, C.
dc.contributor.authorBharathi, M. Vijaya
dc.contributor.authorTANUVAS
dc.date.accessioned2018-04-09T11:50:07Z
dc.date.available2018-04-09T11:50:07Z
dc.date.issued2017-07
dc.descriptionTNV_JPD_2017_41(3)869-873en_US
dc.description.abstractThe present study aimed for the isolation and genotyping of Toxoplasma gondii from small ruminants (sheep and goats). 14 out of 193 tissue samples (either brain and heart) tested positive by MDAT for anti-T. gondii antibodies, were selected and bioassayed, which resulted 4 samples positive for T. gondii after 40 days of post inoculation. Four samples consisting of 3 numbers of sheep and 1 number of goat tissues out of 14 samples detected by B1 PCR, were genotyped at SAG3 locus by nested polymerase chain reaction–restriction fragment length polymorphism technique (nPCR–RFLP). The results of the present study revealed that the four isolates designated as TgShIn19, TgShIn76, TgShIn77 and TgGtIn27 were circulating in small ruminants, were belonged to genotypes of type II (TgShIn19) and type III (TgShIn76, TgShIn77 and TgGtIn27) which are in concordance with the previously reported genotypes from other animal species and further this presumptive results indicating that the genotype II and III could be the predominant in different animal species including birds and humans in India.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810043097
dc.keywordsToxoplasma gondii - PCR–RFLP - Isolation - Genotyping - Small ruminants - South Indiaen_US
dc.language.isoenen_US
dc.pages869–873en_US
dc.publisherJournal Impact‎ Factoren_US
dc.relation.ispartofseries;3
dc.subjectVeterinary Scienceen_US
dc.titleIsolation and characterization of Toxoplasma gondii from small ruminants (sheep and goats) in Chennai City, South Indiaen_US
dc.title.alternativeJournal of Parasitic Diseasesen_US
dc.typeArticleen_US
dc.volume41en_US
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