Isolation and characterization of Toxoplasma gondii from small ruminants (sheep and goats) in Chennai City, South India
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Date
2017-07
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Abstract
The present study aimed for the isolation and
genotyping of Toxoplasma gondii from small ruminants
(sheep and goats). 14 out of 193 tissue samples (either
brain and heart) tested positive by MDAT for anti-T. gondii
antibodies, were selected and bioassayed, which resulted 4
samples positive for T. gondii after 40 days of post inoculation.
Four samples consisting of 3 numbers of sheep and
1 number of goat tissues out of 14 samples detected by B1
PCR, were genotyped at SAG3 locus by nested polymerase
chain reaction–restriction fragment length polymorphism
technique (nPCR–RFLP). The results of the present study
revealed that the four isolates designated as TgShIn19,
TgShIn76, TgShIn77 and TgGtIn27 were circulating in
small ruminants, were belonged to genotypes of type II
(TgShIn19) and type III (TgShIn76, TgShIn77 and
TgGtIn27) which are in concordance with the previously
reported genotypes from other animal species and further
this presumptive results indicating that the genotype II and
III could be the predominant in different animal species
including birds and humans in India.
Description
TNV_JPD_2017_41(3)869-873
Keywords
Veterinary Science