STUDY OF COMBINED EFFICACY OF SYNTHESIZED DOUBLE STRANDED RNA (dsRNA) SPECIFIC TO STRUCTURAL AND NON-STRUCTURAL GENES OF WSSV IN PROTECTING LITOPENAEUS VANNAMEI FROM WSSV INFECTION
Loading...
![Thumbnail Image](assets/images/Item.jpg)
Date
2019
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
RNA interference (RNAi) is a potential strategy to control shrimp viral diseases,
including the white spot disease (WSD) caused by White Spot Syndrome Virus (WSSV).
Although a significant progress has been achieved on dsRNA mediated anti-virus strategy
development, there is still no treatment available to control spread of the disease. This
investigation has mainly focused on utilizing viral structural and non-structural proteins
subunit vaccines to protect the host. In the present study the protective and therapeutic
efficacy of double-stranded RNAs (dsRNAs) targeting structural (VP281) and
nonstructural gene (RR2) of WSSV singly and in combination in controlling viral
multiplication in Litopenaeus vannamei was investigated by intramuscular injection. The
suppression effects of shrimp mortality by either combined dsRNAs of RR2 and VP281 or
dsRNA-RR2 & VP281 alone was monitored for 15 days after viral challenge in both
protective and therapeutic experiments. Targeting the combination of RR2 and VP281
genes provided better protection (90% survival) compared to RR2 (80%) and VP281
(70%) in protective experiment indicating that targeting a combination of RR2 and VP281
would be effective in limiting WSSV multiplication. In therapeutic experiment, 80% of
shrimp survivals were detected from combined dsRNAs treatment, while RR2, VP281
showed survival rates of 70% and 60 % respectively, suggesting that the combination of
dsRNAs can therapeutically clear existing WSSV in shrimp after a relatively short time of
injecting.