SUPPLEMENTATION OF OLIVE AND ALMOND OIL IN EXTENDERS FOR PRESERVATION OF SEMEN IN NARI SUWARNA STRAIN OF SHEEP
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Date
2018-07
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KARNATAKA VETERINARY ANIMAL AND FISHERIES SCIENCE'SUNIVERSITY, BIDAR-585226
Abstract
The study was carried out to evaluate the effect of supplementation of almond oil
(AO) and olive oil (OO) in Skim milk (SM) and Tris egg yolk (TEY) extenders, on sperm
quality parameters of the NARI Suwarna ram semen refrigerated for 72 h. Semen collected
from five mature NARI Suwarna rams was pooled and diluted to a final concentration of
0.8 x 109 spermatozoa/ml in SM and TEY extenders supplemented with AO and OO at
0.25% v/v (SM, SMAO, SMOO, TEY, TEYAO, TEYOO). Semen was then stored at 5°C
and several sperm motility and velocity parameters were assessed by Computer assisted
semen analysis (CASA) at 0, 24, 48 and 72h of storage. Randomized Block Design (RBD)
was used to test the significant difference between extenders. Extenders of TEY (TEY,
TEYAO and TEYOO) were significantly (P<0.05) better as compared to SM, SMAO and
SMOO. Supplemented skim milk extenders (SMAO and SMOO) preserved motility and
velocity parameters better compared to plain SM extender. Addition of olive oil (0.25%)
in TEY extender had beneficial effect in preserving rapid progressive sperm motility
percentage better than in other extenders and sperm curvilinear velocity (VCL) improved
after addition of almond oil and olive oil in TEY extender. Assessment of sperm motility
and velocity parameters by CASA revealed TEY as better extender for preservation of
refrigerated NARI Suwarna ram semen for 72 h.
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