PLANT REGENERATION VIA ORGANOGENESIS FROM MATURE TISSUES OF CITRUS ROOTSTOCK HYBRIDS
| dc.contributor.advisor | A. K. Dubey | |
| dc.contributor.author | SANDEEP | |
| dc.date.accessioned | 2019-11-13T06:44:21Z | |
| dc.date.available | 2019-11-13T06:44:21Z | |
| dc.date.issued | 2019 | |
| dc.description | T-10129 | en_US |
| dc.description.abstract | In the current work, efforts were made to examine culture of nodal explants derived from the mature tissues of field grown two citrus rootstock hybrids CRH-18-2/15 (RLC-6 x pomeroy) and CRH-20-2/15 (small fruited mandarin x Troyer citrange). Additionally, effect of explant type, media supplement and ethylene inhibitors on shoot and root induction were evaluated in citrus rootstock hybrid CRH-18-2/15. Also tested clonal homogeneity of in vitro raised plants using Simple Sequence Repeat (SSR) markers. Results on basic media, explant type and genotype exhibited higher responsive explants (100%) and bud proliferation rate (97.78% and 95.56%) when basal and apical zone segments of CRH-20-2/15 were cultured on DKW medium, but in hybrid CRH18-2/15, higher responsive plants (100%) and bud proliferation (100% and 98.33%) was observed while apical and basal nodal segments inoculated on MT and GB-5 media, respectively. In CRH-20-2/15, highest shoot length (17.67 mm) was measured with middle zone segments when cultured on MT medium, while in other hybrid, higher shoot length was found when middle zone segment cultured either on MT (10.67 mm) or DKW (8.00 mm) medium. In fact, number of leaves/ regenerated explant in CRH20-2/15 hybrid was counted more with middle zone segments inoculated on MT medium but in CRH-18-2/15, it was found higher with apical and basal zone segment while cultured on DKW medium. Earliest shoot induction at 6th day of inoculation was observed with all nodal zones, while cultured on MT medium CRH-20-2/15, though shoot induction was completed within 15 days after inoculation. Media supplement also indicated significant difference in shoot induction of hybrid CRH-18-2/15. DKW medium supplemented either with 2ip or BA along with GA3 and NAA produced satisfactorily results for shoot induction. Higher bud proliferation rate (91.54% and 73.97%) was noticed with apical and middle zone segments, respectively, while cultured on DKW medium supplemented with BA, GA3 and NAA. More number of regenerated explants were found with CoCl2, while silver thiosulfate promoted bud proliferation rate, shoot length and number of buds per regenerated explant. Combined application of both CoCl2 and STS was found to be toxic for shoot induction in hybrid CRH-18-2/15. As judged by SSR primers, all the in vitro raised plants showed 100% uniformity among themselves and with the mother plant. 92 Keywords: Regeneration, explant, basic media, segment | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810135197 | |
| dc.keywords | Regeneration, explant, basic media, segment | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | DIVISION OF FRUITS AND HORTICULTURAL TECHNOLOGY INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI | en_US |
| dc.sub | Fruit Science | en_US |
| dc.subject | null | en_US |
| dc.theme | PLANT REGENERATION VIA ORGANOGENESIS FROM MATURE TISSUES OF CITRUS ROOTSTOCK HYBRIDS | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | PLANT REGENERATION VIA ORGANOGENESIS FROM MATURE TISSUES OF CITRUS ROOTSTOCK HYBRIDS | en_US |
| dc.type | Thesis | en_US |