PLANT REGENERATION VIA ORGANOGENESIS FROM MATURE TISSUES OF CITRUS ROOTSTOCK HYBRIDS
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Date
2019
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DIVISION OF FRUITS AND HORTICULTURAL TECHNOLOGY INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI
Abstract
In the current work, efforts were made to examine culture of nodal explants derived
from the mature tissues of field grown two citrus rootstock hybrids CRH-18-2/15
(RLC-6 x pomeroy) and CRH-20-2/15 (small fruited mandarin x Troyer citrange).
Additionally, effect of explant type, media supplement and ethylene inhibitors on shoot
and root induction were evaluated in citrus rootstock hybrid CRH-18-2/15. Also tested
clonal homogeneity of in vitro raised plants using Simple Sequence Repeat (SSR)
markers. Results on basic media, explant type and genotype exhibited higher responsive
explants (100%) and bud proliferation rate (97.78% and 95.56%) when basal and apical
zone segments of CRH-20-2/15 were cultured on DKW medium, but in hybrid CRH18-2/15, higher responsive plants (100%) and bud proliferation (100% and 98.33%)
was observed while apical and basal nodal segments inoculated on MT and GB-5
media, respectively. In CRH-20-2/15, highest shoot length (17.67 mm) was measured
with middle zone segments when cultured on MT medium, while in other hybrid, higher
shoot length was found when middle zone segment cultured either on MT (10.67 mm)
or DKW (8.00 mm) medium. In fact, number of leaves/ regenerated explant in CRH20-2/15 hybrid was counted more with middle zone segments inoculated on MT
medium but in CRH-18-2/15, it was found higher with apical and basal zone segment
while cultured on DKW medium. Earliest shoot induction at 6th day of inoculation was
observed with all nodal zones, while cultured on MT medium CRH-20-2/15, though
shoot induction was completed within 15 days after inoculation. Media supplement also
indicated significant difference in shoot induction of hybrid CRH-18-2/15. DKW
medium supplemented either with 2ip or BA along with GA3 and NAA produced
satisfactorily results for shoot induction. Higher bud proliferation rate (91.54% and
73.97%) was noticed with apical and middle zone segments, respectively, while
cultured on DKW medium supplemented with BA, GA3 and NAA. More number of
regenerated explants were found with CoCl2, while silver thiosulfate promoted bud
proliferation rate, shoot length and number of buds per regenerated explant. Combined
application of both CoCl2 and STS was found to be toxic for shoot induction in hybrid
CRH-18-2/15. As judged by SSR primers, all the in vitro raised plants showed 100%
uniformity among themselves and with the mother plant.
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Keywords: Regeneration, explant, basic media, segment
Description
T-10129
Keywords
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