MORPHOLOGICAL AND MOLECULAR DIVERSITY IN ISOLATED MUTANT LINES AND DIFFERENT VARIETIES OF BAMBARA GROUNDNUT [Vigna subterranea (L.) Verdc.]
dc.contributor.advisor | Nandini, R | |
dc.contributor.author | MANU, M. D | |
dc.date.accessioned | 2017-09-12T06:18:58Z | |
dc.date.available | 2017-09-12T06:18:58Z | |
dc.date.issued | 2016-07-21 | |
dc.description.abstract | Bambara groundnut (Vigna subterranea (L.) Verdc.) is one of the most neglected and underutilized pulse crop grown mostly in the semi-arid tropics of the African continent. The present investigation was undertaken to detect possible genetic diversity among 46 stabilized mutant lines of Bambara groundnut genotypes developed using gamma rays based on morphological diversity and molecular diversity using RAPD markers was studied during kharif 2015 in the Department of Genetics and Plant Breeding, UAS, GKVK and MRS, Hebbala, Bengaluru. Forty six genotypes of Bambara groundnut were subjected to genetic divergence using D2 statistics. All the 46 genotypes were grouped into six clusters. Cluster I was largest with twenty three genotypes. Intra-cluster and inter-cluster D2 values ranged from 0 to 26.56 and 30.24 to 103.16, respectively. The Maximum inter cluster distance was observed between cluster III and VI. The maximum contribution towards genetic divergence was due to number of leaves per plant (64.53 %). Genotypes from cluster II with VI, cluster I with VI and cluster I with V may serve as potential parents for hybridization programme. Genetic diversity was also evaluated using Random Amplified Polymorphic DNA (RAPD) markers. RAPDs revealed considerable levels of polymorphism among genotypes. The per cent polymorphism ranged from 0 % to 100 % with an average of 71.42 %. The construction of genetic relationships using cluster analysis grouped the 46 genotypes into two major clusters. RAPDs are useful for the genetic diversity studies in V. subterranea and can identify variation within genotypes. | en_US |
dc.identifier.other | Th-11377 | |
dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810030904 | |
dc.language.iso | en | en_US |
dc.pages | 84 | en_US |
dc.publisher | UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU | en_US |
dc.sub | Genetics and Plant Breeding | en_US |
dc.subject | null | en_US |
dc.theme | MORPHOLOGICAL AND MOLECULAR DIVERSITY IN ISOLATED MUTANT LINES AND DIFFERENT VARIETIES | en_US |
dc.these.type | M.Sc | en_US |
dc.title | MORPHOLOGICAL AND MOLECULAR DIVERSITY IN ISOLATED MUTANT LINES AND DIFFERENT VARIETIES OF BAMBARA GROUNDNUT [Vigna subterranea (L.) Verdc.] | en_US |
dc.type | Thesis | en_US |