MORPHOLOGICAL AND MOLECULAR DIVERSITY IN ISOLATED MUTANT LINES AND DIFFERENT VARIETIES OF BAMBARA GROUNDNUT [Vigna subterranea (L.) Verdc.]
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Date
2016-07-21
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Bambara groundnut (Vigna subterranea (L.) Verdc.) is one of the most
neglected and underutilized pulse crop grown mostly in the semi-arid tropics of the
African continent. The present investigation was undertaken to detect possible genetic
diversity among 46 stabilized mutant lines of Bambara groundnut genotypes
developed using gamma rays based on morphological diversity and molecular
diversity using RAPD markers was studied during kharif 2015 in the Department of
Genetics and Plant Breeding, UAS, GKVK and MRS, Hebbala, Bengaluru. Forty six
genotypes of Bambara groundnut were subjected to genetic divergence using D2
statistics. All the 46 genotypes were grouped into six clusters. Cluster I was largest
with twenty three genotypes. Intra-cluster and inter-cluster D2 values ranged from 0 to
26.56 and 30.24 to 103.16, respectively. The Maximum inter cluster distance was
observed between cluster III and VI. The maximum contribution towards genetic
divergence was due to number of leaves per plant (64.53 %). Genotypes from cluster
II with VI, cluster I with VI and cluster I with V may serve as potential parents for
hybridization programme. Genetic diversity was also evaluated using Random
Amplified Polymorphic DNA (RAPD) markers. RAPDs revealed considerable levels
of polymorphism among genotypes. The per cent polymorphism ranged from 0 % to
100 % with an average of 71.42 %. The construction of genetic relationships using
cluster analysis grouped the 46 genotypes into two major clusters. RAPDs are useful
for the genetic diversity studies in V. subterranea and can identify variation within
genotypes.
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