Development of efficient plant regeneration system in Kinnow mandarin(Citrus nobilis Lour × Citrus deliciosa Tenora) for genetic transformation
| dc.contributor.advisor | Chauhan, V. S. | |
| dc.contributor.author | Sataveer | |
| dc.date.accessioned | 2017-08-10T04:58:06Z | |
| dc.date.available | 2017-08-10T04:58:06Z | |
| dc.date.issued | 2008 | |
| dc.description.abstract | The present study was carried out to develop an efficient plant regeneration system in Kinnow mandarin (Citrus nobilis Lour X Citrus deliciosa Tenora) for genetic transformation. Direct regeneration of shoots and roots without intervention of callus from epicotyl segments collected from in vitro raised seedling and shoot tips collected from in vivo raised plants was observed on MS basal medium supplemented with different cytokinins and auxins. The MS+BAP 1.Omg/l was recorded to be the best treatment for shoot proliferation in in vitro epicotyl explants and in vivo shoot tip explants. The MS + BAP 1.0 mg/1 was observed to be the best treatment for shoot proliferation in in vitro epicotyl explants and shoot tip explants in case of parameters like percent regeneration, number of shoots per explant, shoot length followed by MS+Kin l.0 mg/l. The number of shoots per explant ranged, from 1.20 to 4.20 in epicotyl and 1.0 to 4.20 in shoot tips. The maximum length of shoot was recorded 3.20 cm in case of In vitro epicotyl segments and 2.60 cm in shoot tips on the MS+BAP 1mg/l. In case of rooting medium ½ MS+IBA 1.Omg/l was found to be best for different aspects of root differentiation like number of days required for root initiation (13), per cent rooting (94%) number of roots/planted (4.20), root length (4. 1 cm). | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810028212 | |
| dc.keywords | Regression, Kinnow mandarin, Citrus, ln vitro epicotyl, Shoot tips growth regulators | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | CCSHAU | en_US |
| dc.sub | Horticulture | en_US |
| dc.theme | Development of efficient plant regeneration system in Kinnow mandarin(Citrus nobilis Lour × Citrus deliciosa Tenora) for genetic transformation | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Development of efficient plant regeneration system in Kinnow mandarin(Citrus nobilis Lour × Citrus deliciosa Tenora) for genetic transformation | en_US |
| dc.type | Thesis | en_US |