Development of efficient plant regeneration system in Kinnow mandarin(Citrus nobilis Lour × Citrus deliciosa Tenora) for genetic transformation
Loading...
Date
2008
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CCSHAU
Abstract
The present study was carried out to develop an efficient plant
regeneration system in Kinnow mandarin (Citrus nobilis Lour X Citrus deliciosa Tenora)
for genetic transformation. Direct regeneration of shoots and roots without
intervention of callus from epicotyl segments collected from in vitro raised seedling
and shoot tips collected from in vivo raised plants was observed on MS basal medium
supplemented with different cytokinins and auxins. The MS+BAP 1.Omg/l was
recorded to be the best treatment for shoot proliferation in in vitro epicotyl explants
and in vivo shoot tip explants. The MS + BAP 1.0 mg/1 was observed to be the best
treatment for shoot proliferation in in vitro epicotyl explants and shoot tip explants in
case of parameters like percent regeneration, number of shoots per explant, shoot
length followed by MS+Kin l.0 mg/l. The number of shoots per explant ranged, from
1.20 to 4.20 in epicotyl and 1.0 to 4.20 in shoot tips. The maximum length of shoot
was recorded 3.20 cm in case of In vitro epicotyl segments and 2.60 cm in shoot tips
on the MS+BAP 1mg/l. In case of rooting medium ½ MS+IBA 1.Omg/l was found to be
best for different aspects of root differentiation like number of days required for root
initiation (13), per cent rooting (94%) number of roots/planted (4.20), root length (4. 1
cm).