Immunogenetic and biochemical profiling of Gaddi dogs

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dc.contributor.advisorSharma, Mandeep
dc.contributor.authorYadav, Priyanka
dc.date.accessioned2023-02-23T10:03:30Z
dc.date.available2023-02-23T10:03:30Z
dc.date.issued2023-01-24
dc.description.abstractGaddi dog is a generic term deriving its name from the nomadic shepherds referred to as Gaddis tribe (parts of Himachal Pradesh, Uttarakhand, J&K); who rear these dogs primarily to protect and guard their sheep and goats. It is a matter of great concern that this important germplasm is unknown to the masses and not recognized by any Kennel Club of India. This unique canine germplasm, therefore, needs to be documented and registered. The present study was designed to study the blood and haematological profiles; and parameters related to the innate immunity of Gaddi dogs that may help in the characterisation and registration of these dogs to a defined breed. The average physiological values for rectal temperature (101.4 ± 0.2 °F), respiratory rate (25.6 ± 0.60 breaths per min.), and heart rate (127.3 ± 1.20 beats per minute) were recorded. The values recorded for haematology and biochemical parameters from seven Gaddi dogs were within the normal range as has been reported for dogs of some other breeds. The mean percent values of major lymphocyte subsets were 44.50, 25.03, and 10.30 for T-helper cells (CD4+), cytotoxic T cells (CD8+) and B lymphocytes (CD21+), respectively. The respiratory burst activity of PMNCs (neutrophils) as measured by Nitroblue tetrazolium test and the DCFH-DA probe-based assay revealed around two-fold increase. Ferric reducing the ability of plasma assay showed higher FRAP values for Gaddi dogs (0.183-0.234 Fe2+ µmoles per ml) when compared with diseased dogs (0.172-0.201 Fe2+ µmoles per ml) of other breeds. The complement haemolytic activity (CH50) of Gaddi dogs was found to be in the range of (19-50.9 CH50 units) and the mean was 27.2 CH50 units. These values for dogs of some other breeds were in the range of 22.7-37.8 CH50 units. The expressions and sequences variation of two major antimicrobial peptides, CBD-103 and K9CATH were also studied. The expression of both CBD-103 and K9CATH were noticed in blood, oral and nasal mucosa. A 3-base pair deletion resulting in the loss of Glycine at position 23 of CBD-103 has been associated with black coat colour in certain breeds of dogs. The result of this study found that the coat colour in studied Gaddi dogs was independent of G23 deletion in CBD-103 and it was not associated with black coat colour in these dogs. This study also reported multiple alleles of K9CATH in Gaddi dogs.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810194320
dc.keywordsDogs, Gaddi Dogs, Immunogenetic profiling, Biochemical profiling, Canine germplasmen_US
dc.language.isoEnglishen_US
dc.pages89en_US
dc.publisherCSK HPKV, Palampuren_US
dc.subVeterinary Microbiologyen_US
dc.themeTo study blood cell phenotypes and biochemical profiles; and to study some important parameters related to innate immunityen_US
dc.these.typeM.V.Sc.en_US
dc.titleImmunogenetic and biochemical profiling of Gaddi dogsen_US
dc.typeThesisen_US
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