SSR markers based genetic diversity studies in Valeriana jatamansi Jones.

Simple item page
dc.contributor.advisorSharma, Ram Kumar
dc.contributor.authorKumari, Nirupma
dc.date.accessioned2022-02-10T07:27:23Z
dc.date.available2022-02-10T07:27:23Z
dc.date.issued2021-10-28
dc.description.abstractValeriana jatamansi Jones. is a perennial, tetraploid (2n = 4x = 32), gynodioecious herb from family Valerianaceae. It is native to the Himalayan region, distributed from an altitude range of 1,000 to 3,000 m asl (above sea level) and in India, it is mainly distributed in the northern and north-eastern states. Due to great economic importance for its unique medicinal, flavour and aromatic properties the species have been subjected to great habitat degradation and fragmentation by the illegal trade. Additionally, plant also lacks any molecular genetics efforts mainly because of the non-availability of genome-wide molecular marker resources. In this study, a total of 9,988 SSR containing sequences were derived with maximum abundance of direpeats (~48%) followed by tri-repeats (~32%). Furthermore, synthesis and validation of 103 functionally SSR markers identified 51 polymorphic markers. Extrapolation of highly informative 10 SSR markers for genotyping 100 individuals representing ten diverse geographical populations suggests wide utilization of these markers in large scale fingerprinting studies of V. jatamansi germplasm. Neighbor- joining based hierarchical clustering and Bayesian structure clustering analysis corresponded each other and grouped 100 genotypes into two major clusters suggesting that there are two major genetic pools operating in Himachal Pradesh. Further, AMOVA analysis suggests maximum diversity exists within population (75%). Moderate genetic differentiation (Fst = 0.14) with significant gene flow (Nm = 1.79) may be attributed to natural bottlenecks and various anthropogenic activities in the region. Microsatellite markers identified in this study can be categorized as “Informative Markers” which can be further used for genotyping, genome mapping, evolutionary studies and implementation conservation plans for sustainable utilization of V. jatamansi.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810182025
dc.keywordsExtrapolation,genotypes, formation,germplasm,en_US
dc.language.isoEnglishen_US
dc.pages58en_US
dc.publisherPalampuren_US
dc.subAgricultural Biochemistryen_US
dc.themeValidation,assessment of polymorphic potential of sequence based SSR markers and Utilization of polymorphic SSR markers for genetic diversity assessment in Valeriana jatamansi.en_US
dc.these.typeM.Scen_US
dc.titleSSR markers based genetic diversity studies in Valeriana jatamansi Jones.en_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Pote Tushar Diliprao Msc.Agr.Bio-technology.pdf
Size:
2.15 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses