SSR markers based genetic diversity studies in Valeriana jatamansi Jones.
dc.contributor.advisor | Sharma, Ram Kumar | |
dc.contributor.author | Kumari, Nirupma | |
dc.date.accessioned | 2022-02-10T07:27:23Z | |
dc.date.available | 2022-02-10T07:27:23Z | |
dc.date.issued | 2021-10-28 | |
dc.description.abstract | Valeriana jatamansi Jones. is a perennial, tetraploid (2n = 4x = 32), gynodioecious herb from family Valerianaceae. It is native to the Himalayan region, distributed from an altitude range of 1,000 to 3,000 m asl (above sea level) and in India, it is mainly distributed in the northern and north-eastern states. Due to great economic importance for its unique medicinal, flavour and aromatic properties the species have been subjected to great habitat degradation and fragmentation by the illegal trade. Additionally, plant also lacks any molecular genetics efforts mainly because of the non-availability of genome-wide molecular marker resources. In this study, a total of 9,988 SSR containing sequences were derived with maximum abundance of direpeats (~48%) followed by tri-repeats (~32%). Furthermore, synthesis and validation of 103 functionally SSR markers identified 51 polymorphic markers. Extrapolation of highly informative 10 SSR markers for genotyping 100 individuals representing ten diverse geographical populations suggests wide utilization of these markers in large scale fingerprinting studies of V. jatamansi germplasm. Neighbor- joining based hierarchical clustering and Bayesian structure clustering analysis corresponded each other and grouped 100 genotypes into two major clusters suggesting that there are two major genetic pools operating in Himachal Pradesh. Further, AMOVA analysis suggests maximum diversity exists within population (75%). Moderate genetic differentiation (Fst = 0.14) with significant gene flow (Nm = 1.79) may be attributed to natural bottlenecks and various anthropogenic activities in the region. Microsatellite markers identified in this study can be categorized as “Informative Markers” which can be further used for genotyping, genome mapping, evolutionary studies and implementation conservation plans for sustainable utilization of V. jatamansi. | en_US |
dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810182025 | |
dc.keywords | Extrapolation,genotypes, formation,germplasm, | en_US |
dc.language.iso | English | en_US |
dc.pages | 58 | en_US |
dc.publisher | Palampur | en_US |
dc.sub | Agricultural Biochemistry | en_US |
dc.theme | Validation,assessment of polymorphic potential of sequence based SSR markers and Utilization of polymorphic SSR markers for genetic diversity assessment in Valeriana jatamansi. | en_US |
dc.these.type | M.Sc | en_US |
dc.title | SSR markers based genetic diversity studies in Valeriana jatamansi Jones. | en_US |
dc.type | Thesis | en_US |
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