Efficacy of multifaceted microbes against Fusarial wilt of chickpea.

dc.contributor.advisorDevi, R. K. Tombisana
dc.contributor.authorPatel, Kailash
dc.date.accessioned2023-02-24T08:01:27Z
dc.date.available2023-02-24T08:01:27Z
dc.date.issued2022-09
dc.description.abstractChickpea is a leguminous crop rich in protein and minerals. Fusarial wilt is a major disease causing both qualitative and quantitative loss up to 100% worldwide. In the present investigation Fusarium oxysporum f.sp. ciceris (FOC) was isolated from diseased samples showing typical disease symptoms. The pathogen was confirmed based on morphology, pathogenicity test and molecular characterization using two different primers ITS4 and ITS6 and EF1 and EF2. Four fungicides were evaluated against growth of FOC. Among them carbendazim and propiconazole could completely inhibit at all the concentrations. Fifty endophytes were isolated from five leguminous plants. Out of which eight endophytes were selected showing more than 60% inhibition of growth of pathogen. Out of which four endophytes (ECP1, ECP8, Egg8, EMP4) as Bacillus cereus, two (ECP10, EGN2) as Bacillus subtilis, ECP5 as Enterobacter sp., ESB2 as Bacillus amyloliquifaciens, were identified based on 16S rRNA sequencing. Six microbial consortia containing four endophytes in each consortium were formulated and evaluated both in vitro and in vivo conditions. Among all the treatments, T5 (ECP8+ECP10+ ESB2+EGN2) was found superior in maximum seed germination (100%) and vigour index (2805.56) and lowest disease incidence of 8.06 and 9.33 % at 30 and 60 days after sowing to other treatments. T5 showed significantly increased plant growth and yield upto 19.82 q/ha than other treatments.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810194445
dc.keywordsChickpea, Fusarial wilt, Bacillus, Endophytes, Molecular characterization.en_US
dc.language.isoEnglishen_US
dc.pages87p.en_US
dc.publisherCollege of Post Graduate Studies in Agricultural Sciences, CAU-Imphal, Umiamen_US
dc.subPlant Pathologyen_US
dc.themeAcademic Researchen_US
dc.these.typeM.Scen_US
dc.titleEfficacy of multifaceted microbes against Fusarial wilt of chickpea.en_US
dc.typeThesisen_US
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