Efficacy of multifaceted microbes against Fusarial wilt of chickpea.
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Date
2022-09
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College of Post Graduate Studies in Agricultural Sciences, CAU-Imphal, Umiam
Abstract
Chickpea is a leguminous crop rich in protein and minerals. Fusarial wilt is a major disease causing both qualitative and quantitative loss up to 100% worldwide. In the present investigation Fusarium oxysporum f.sp. ciceris (FOC) was isolated from diseased samples showing typical disease symptoms. The pathogen was confirmed based on morphology, pathogenicity test and molecular characterization using two different primers ITS4 and ITS6 and EF1 and EF2. Four fungicides were evaluated against growth of FOC. Among them carbendazim and propiconazole could completely inhibit at all the concentrations. Fifty endophytes were isolated from five leguminous plants. Out of which eight endophytes were selected showing more than 60% inhibition of growth of pathogen. Out of which four endophytes (ECP1, ECP8, Egg8, EMP4) as Bacillus cereus, two (ECP10, EGN2) as Bacillus subtilis, ECP5 as Enterobacter sp., ESB2 as Bacillus amyloliquifaciens, were identified based on 16S rRNA sequencing. Six microbial consortia containing four endophytes in each consortium were formulated and evaluated both in vitro and in vivo conditions. Among all the treatments, T5 (ECP8+ECP10+ ESB2+EGN2) was found superior in maximum seed germination (100%) and vigour index (2805.56) and lowest disease incidence of 8.06 and 9.33 % at 30 and 60 days after sowing to other treatments. T5 showed significantly increased plant growth and yield upto 19.82 q/ha than other treatments.