IDENTIFICATION OF DROUGHT TOLERANT RICE (Oryza sativa L.) GENOTYPES FOR RAINFED LOWLAND ECOLOGY USING MICROSATELLITE MARKERS LINKED TO DROUGHT
| dc.contributor.advisor | Singh, D.N. | |
| dc.contributor.author | Kar, Sonali Kar | |
| dc.date.accessioned | 2017-06-20T07:14:54Z | |
| dc.date.available | 2017-06-20T07:14:54Z | |
| dc.date.issued | 2014 | |
| dc.description | IDENTIFICATION OF DROUGHT TOLERANT RICE (Oryza sativa L.) GENOTYPES FOR RAINFED LOWLAND ECOLOGY USING MICROSATELLITE MARKERS LINKED TO DROUGHT | en_US |
| dc.description.abstract | Drought is referred to as low-moisture stress or is a form of abiotic stress which is a challenge posed by the environment to the survival and productivity of a crop that occupies a large area. The development of drought tolerant rice varieties is considered a feasible option to counteract drought stress. However the breeding efforts to develop varieties with improved productivity are being hindered due to lack of understanding of inheritance of drought tolerance and poor understanding of physiological basis of yield under water stress. The aim of the present study was to select drought tolerant genotypes from the F6 population of a cross between BPT-5204 (drought susceptible) and Sahbhagi Dhan (drought tolerant) in lowland ecology by the use of morphological markers and MAS (Marker Assisted Selection) with SSRs available in the public domain. Screening of Eight Hundred and Ten Recombinant Inbred Lines (RILs) for drought was carried out under rainout condition. Stress was imposed for 5 weeks at vegetative stage of the crop to screen for drought tolerant lines. Experimental results based on the observations of leaf rolling, leaf drying and recovery identified 140 highly drought tolerant lines. Mean, variability and correlation studies were made based on the observations recorded for various morphological and yield attributing characters. As per the statistical analysis there was substantial variation among the traits. Further, 20 phenotypically highly drought tolerant RILs were selected for root studies and molecular studies using microsatellite markers linked to drought. 19 SSR primers were used to screen for parental polymorphism, out of which 6 showed polymorphism. The drought tolerant lines confirmed using these six polymorphic SSRs were line numbers 4, 122, 125, 135, 137, 152, 161, 162, 165, 179, 180, 181, 193, 194, 205, 418, 586, 808, 809 and 810. Among the 20 drought tolerant genotypes, the maximum yielding lines were line number 4, 137, 181, 193 and 808 with medium maturity duration. Molecular studies revealed the presence of maximum of 4 QTLs for drought in a single genotype. However, the drought QTLs detected were on chromosomes 1, 3, 4, 5, 6 and 7. These QTLs were linked to various characters like plant height, panicle length, grain yield, root thickness, root length, root volume, root to shoot ratio, days to 50% flowering, biomass, harvest index and test weight. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810022044 | |
| dc.keywords | IDENTIFICATION OF DROUGHT TOLERANT RICE (Oryza sativa L.) GENOTYPES FOR RAINFED LOWLAND ECOLOGY USING MICROSATELLITE MARKERS LINKED TO DROUGHT | en_US |
| dc.language.iso | en_US | en_US |
| dc.pages | 97 | en_US |
| dc.publisher | Birsa Agricultural University, Kanke, Ranchi, Jharkhand | en_US |
| dc.sub | Genetics and Plant Breeding | en_US |
| dc.subject | null | en_US |
| dc.theme | IDENTIFICATION OF DROUGHT TOLERANT RICE (Oryza sativa L.) GENOTYPES FOR RAINFED LOWLAND ECOLOGY USING MICROSATELLITE MARKERS LINKED TO DROUGHT | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | IDENTIFICATION OF DROUGHT TOLERANT RICE (Oryza sativa L.) GENOTYPES FOR RAINFED LOWLAND ECOLOGY USING MICROSATELLITE MARKERS LINKED TO DROUGHT | en_US |
| dc.type | Thesis | en_US |