Antigenic Profile of Excretory / Secretory Antigens of Immature Paramphistomes

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dc.contributor.authorVijayashanthi, R.
dc.contributor.authorSoundararajan, C.
dc.contributor.authorRaman, M.
dc.contributor.authorKumar, T.M.A. Senthil
dc.contributor.authorTANUVAS
dc.date.accessioned2019-07-02T08:53:57Z
dc.date.available2019-07-02T08:53:57Z
dc.date.issued2019-06
dc.descriptionTNV_IVJ_Jun2019_96(06)_29-31en_US
dc.description.abstractThe present study focused on characterization of excretory/secretory antigens of pathogenic immature paramphistomes which could be used for immunodiagnosis of immature paramphistomosis in sheep. The immature flukes were collected from the duodenum of sheep at Perambur slaughter house, Chennai. The flukes were incubated in 10 mM phosphate buffered saline (10 flukes/6ml) at 28.3°C for overnight. The protein concentration varied from 0.236 mg/ml to 0.314 mg/ml using bicinchoninic acid (BCA) method. SDS-PAGE (12%) analysis of the total excretory/secretory antigen of immature flukes revealed 4 immunodominant bands in the range of 10 to 20 kDa which might be used for immunodiagnosis of immature paramphistomosis in sheep.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810111223
dc.keywordsVeterinary Science, Veterinary Parasitology, Excretory/Secretory antigens, Immature paramphistomes, SDS-PAGEen_US
dc.language.isoen_USen_US
dc.pages29-31en_US
dc.publisherTANUVAS, Chennaien_US
dc.relation.ispartofseries;06
dc.subjectVeterinary Scienceen_US
dc.subjectVeterinary Parasitologyen_US
dc.titleAntigenic Profile of Excretory / Secretory Antigens of Immature Paramphistomesen_US
dc.title.alternativeIndian Veterinary Journalen_US
dc.typeArticleen_US
dc.volume96en_US
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