Antigenic Profile of Excretory / Secretory Antigens of Immature Paramphistomes
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Date
2019-06
Journal Title
Journal ISSN
Volume Title
Publisher
TANUVAS, Chennai
Abstract
The present study focused on characterization
of excretory/secretory antigens of pathogenic
immature paramphistomes which could be
used for immunodiagnosis of immature paramphistomosis
in sheep. The immature flukes
were collected from the duodenum of sheep at
Perambur slaughter house, Chennai. The flukes
were incubated in 10 mM phosphate buffered
saline (10 flukes/6ml) at 28.3°C for overnight.
The protein concentration varied from 0.236
mg/ml to 0.314 mg/ml using bicinchoninic acid
(BCA) method. SDS-PAGE (12%) analysis of the
total excretory/secretory antigen of immature
flukes revealed 4 immunodominant bands in the
range of 10 to 20 kDa which might be used for
immunodiagnosis of immature paramphistomosis
in sheep.
Description
TNV_IVJ_Jun2019_96(06)_29-31
Keywords
Veterinary Science, Veterinary Parasitology