STUDY ON THE EFFICACY OF NISIN AS AN ANTIMICROBIAL AGENT IN PANEER PACKAGING MATERIAL
Loading...
Date
2021-07-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR –
Abstract
The present study was aimed in increasing the shelf life of paneer by incorporating
antibacterial agent nisin to the packaging material. TBC, Coliform, E. coli and Aerobic
spores counts from packaging material were 1.01, 0.80, 0.81 and 0.87 log10 cfu/cm2 and in
paneer samples TBC, Coliforms, Staph. aureus and Aerobic spores counts of 5.19, 3.60,
2.75 and 1.14 log10 cfu/g respectively. Packaging material isolates were identified as
Klebsiella ozaenae, Citrobacter intermedius, E. coli and Bacillus cereus and Paneer
isolates were identified as Micrococcus varians, Citrobacter intermedius, E. coli,
Salmonella sp., Staph. aureus, and Bacillus cereus respectively. Micrococcus varians,
Staph. aureus and Bacillus cereus showed maximum inhibitory area of 374.66, 184.00,
273.66 sq. mm at 200 ppm nisin. The inhibitory area obtained against Micrococcus varians
we're 413.96, 454.45, 341.63 and 56.56 sq.mm; 188.52, 138.48, 74.63 and nil for Staph.
aureus; 245.08, 188.52, 124.14 and 25.14 Sq.mm for Bacillus cereus at 200 ppm nisin of
pH 2.6, 4.6, 6.6 and 8.6. Heat treated nisin for 85 ℃/30 min showed maximum inhibitory
area of 274.25, 93.16, 138.83 sq. mm against Micrococcus varians, Staph. aureus and
Bacillus cereus. Effect of nisin coated films with 50, 100, 150, 200 ppm soaking time on
the survivability of the test organisms revealed that at 200 ppm, the percentage reduction
was more which was about 86.2, 83.5, 83.0 percent for Micrococcus varians, Staph. aureus
and Bacillus cereus. The 200 ppm nisin coated film packed paneer stored at refrigeration
temperature (7±1 ˚C) found acceptable till 12th day during storage.