ASSESSMENT OF IMMUNE RESPONSES TO RABIES VACCINATION IN FREE RANGING DOGS IN BENGALURU
Loading...
Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
The present study was undertaken to assess the effectiveness of ongoing MDV
programme in stray dogs of Bengaluru. In all, 260 sera samples from stray dogs in 26
wards from 8 zones of BBMP were collected for the study and tested by RFFIT and inhouse
iELISA for HI response and Interferon-γ ELISA for CMIR. Overall, 71 per cent
(171/240) samples from 8 zones of BBMP and 65 per cent (13/20) samples from 2 NC
wards revealed adequate IR by RFFIT. By iELISA, 87 per cent (209/240) samples from 8
zones of BBMP and 90 per cent (18/20) samples from 2 NC wards revealed adequate IR.
Overall, the iELISA revealed higher percentage of seroconversion than RFFIT as it
detects the non-neutralizing antibodies in addition to the neutralizing antibodies. Among
8 zones of BBMP, the samples from B. west had highest seroconversion of 86.36 and 100
per cent by RFFIT and iELISA respectively. The Spearman’s rank correlation (r value ≠
0 and p value <0.05) indicated some correlation and kappa value of 0.55 suggested a
moderate agreement between the results of RFFIT and iELISA. The sensitivity and
specificity of iELISA was found to be 100 per cent and 63.30 per cent respectively. The
In-house iELISA can be used in large population surveys instead of RFFIT to overcome
the disadvantages associated with it. The IFN–γ ELISA employed to study CMIR
revealed 50 per cent (120/240) samples from 8 zones of BBMP and 90 per cent (18/20)
samples from 2 NC wards with adequate CMIR. The highest CMIR of 81.82 per cent was
observed in B. west zone. The adequate CMIR in serum samples with RFFIT titres < 0.5
IU/mL indicates the effect of vaccination being done under the ABC-ARV programme
before initiation of MDV in BBMP. A sustained MDV programme enhanced to cover 70
per cent of the dog population will surely aid in elimination of dog-mediated rabies.