IDENTIFICATION OF MOLECULAR MARKERS LINKED TO YELLOW MOSAIC VIRUS RESISTANCE IN BLACKGRAM (Vigna mungo (L).Hepper)

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dc.contributor.advisorANURADHA, CH
dc.contributor.authorJEEVULA NAIK, B
dc.date.accessioned2016-12-28T14:23:14Z
dc.date.available2016-12-28T14:23:14Z
dc.date.issued2015
dc.description.abstractBlackgram (Vigna mungo (L). Hepper) (2n=22) is an excellent source of easily digestible proteins with low flatulence which complements the staple rice diet in Asia. YMD affects many legumes in India and other south Asian countries and is caused by whitefly (BemisiatabaciGenn.) transmitted by Gemini viruses. These Plant viruses are responsible for a significant proportion of crop diseases. Blackgram (Vignamungo (L). Hepper) MYMV is the most destructive viral disease affecting yield potential of blackgram both qualitatively and quantitatively and ability to cause yield loss up to 85%.The advancements in the field of biotechnology and molecular biology such as marker assisted selection and genetic transformation can be utilized in developing MYMV resistant urdbean. In order to identify the markers linked to yellow mosaic virus resistance gene T9 (MYMV resistant) and LBG 759 (MYMV susceptible) parents were crossed to produce F1 and F2 mapping population. Parents P1 and P2, F1 and 112 F2 mapping population were subjected to natural screening to assess their resistance or susceptibility against MYMV. This study revealed that single recessive is governing the inheritance of resistance to MYMV. F2 mapping population segregated gene in 85 susceptible:27 resistant i.e 3:1 ratio showing that resistance to yellow mosaic virus is governed by monogenic recessive gene A total of 59 SSR markers were used to study parental polymorphism. Of these 12 SSR markers were found polymorphic showing 20.30% of polymorphism between the parents. These 12 markers were used to screen the F2 populations to find the markers linked to the resistance gene by bulk segregant analysis. The marker VR9 present on linkage group 8 clearly distinguished resistant and susceptible parents, bulks and ten F2 resistant and susceptible plants indicating that this marker is tightly linked to yellow mosaic virus resistance gene.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/93489
dc.language.isoenen_US
dc.publisherPROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY RAJENDRANAGAR, HYDERABADen_US
dc.relation.ispartofseriesD9876;
dc.subPlant Molecular Biology and Biotechnology
dc.subjectIDENTIFICATION, MOLECULAR, MARKERS, LINKED, YELLOW MOSAIC, VIRUS RESISTANCE, BLACKGRAMen_US
dc.these.typeM.Sc
dc.titleIDENTIFICATION OF MOLECULAR MARKERS LINKED TO YELLOW MOSAIC VIRUS RESISTANCE IN BLACKGRAM (Vigna mungo (L).Hepper)en_US
dc.typeThesisen_US
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