IDENTIFICATION OF MOLECULAR MARKERS LINKED TO YELLOW MOSAIC VIRUS RESISTANCE IN BLACKGRAM (Vigna mungo (L).Hepper)
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Date
2015
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PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY RAJENDRANAGAR, HYDERABAD
Abstract
Blackgram (Vigna mungo (L). Hepper) (2n=22) is an excellent source of easily
digestible proteins with low flatulence which complements the staple rice diet in Asia.
YMD affects many legumes in India and other south Asian countries and is caused by
whitefly (BemisiatabaciGenn.) transmitted by Gemini viruses. These Plant viruses are
responsible for a significant proportion of crop diseases. Blackgram (Vignamungo (L).
Hepper) MYMV is the most destructive viral disease affecting yield potential of blackgram
both qualitatively and quantitatively and ability to cause yield loss up to 85%.The
advancements in the field of biotechnology and molecular biology such as marker
assisted selection and genetic transformation can be utilized in developing MYMV
resistant urdbean.
In order to identify the markers linked to yellow mosaic virus resistance gene T9
(MYMV resistant) and LBG 759 (MYMV susceptible) parents were crossed to produce
F1 and F2 mapping population. Parents P1 and P2, F1 and 112 F2 mapping population
were subjected to natural screening to assess their resistance or susceptibility against
MYMV. This study revealed that single recessive is governing the inheritance of
resistance to MYMV. F2 mapping population segregated gene in 85 susceptible:27
resistant i.e 3:1 ratio showing that resistance to yellow mosaic virus is governed by
monogenic recessive gene
A total of 59 SSR markers were used to study parental polymorphism. Of these
12 SSR markers were found polymorphic showing 20.30% of polymorphism between
the parents. These 12 markers were used to screen the F2 populations to find the markers
linked to the resistance gene by bulk segregant analysis. The marker VR9 present on
linkage group 8 clearly distinguished resistant and susceptible parents, bulks and ten F2
resistant and susceptible plants indicating that this marker is tightly linked to yellow
mosaic virus resistance gene.
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Keywords
IDENTIFICATION, MOLECULAR, MARKERS, LINKED, YELLOW MOSAIC, VIRUS RESISTANCE, BLACKGRAM