MOLECULAR CHARACTERISATION OF AN ILARVIRUS INFECTING APPLE
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Date
2018-07
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UHF,NAUNI
Abstract
ABSTRACT
The present study entitled “Molecular Characterisation of an Ilarvirus infecting
Apple” was conducted in the Department of Plant Pathology, Dr. Yashwant Singh Parmar
University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh during the year
2017. Surveys revealed that the incidence of virus disease in different apple growing areas
of district Shimla during 2017 ranged between 5 to 71 percent. The predominant symptoms
prevalent in most of the cultivars observed were chlorotic, bright yellow discolouration of
the leaves in the form of blotching, mottling, vein banding or yellowing, ringspots, line and
oak leaf patterns. Twenty isolates of apple were collected from different localities. A
detailed study of the symptomatology of ilarviruses in apple revealed the presence of mixed
infection of apple mosaic virus (ApMV) and prunus necrotic ringspot virus (PNRSV) in
most of the trees. These results were further confirmed through serological detection by
using ALP based direct form of DAS-ELISA. However, the prevalence of PNRSV in
ELISA tests was found to be very low in comparison to ApMV. Among all plant parts tested
for ELISA, apple leaves were found to be the most suitable for the detection of Ilarviruses
(ApMV and PNRSV). DAS-ELISA based indexing between April and August was found to
be the most appropriate period for detecting the presence of ApMV and PNRSV in apple.
RT-PCR based molecular detection also resulted in the successful detection of apple mosaic
virus as CP gene of ApMV was amplified using total RNA isolated from apple using ApMV
CP gene specific primers and an amplicon of ~614 bp was obtained. High incidence of
infections calls for an evaluation of the health status of apple nurseries thereby emphasizing
the need for developing a sound certification scheme for the production of virus free
planting material of apple.
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