MOLECULAR DETECTION OF SUGARCANE YELLOW LEAF VIRUS AND FIELD REACTION OF SUGARCANE GENOTYPES FOR THEIR RESISTANCE
| dc.contributor.advisor | PRAMEELA, A. | |
| dc.contributor.author | BASAVARAJ, S. | |
| dc.date.accessioned | 2017-06-21T05:49:35Z | |
| dc.date.available | 2017-06-21T05:49:35Z | |
| dc.date.issued | 2009-07-15 | |
| dc.description.abstract | Survey conducted to assess the incidence of yellow leaf disease of sugarcane in Cauvery command area of Southern Karnataka indicated the occurrence of disease incidence up to 35.3 per cent. Among the three districts, highest disease incidence of 35.3 per cent was in Mandya district and lowest incidence was recorded in Chamarajnagar district (10.5 %). A fluorescent microscopic technique using aniline blue dye was standardized for detection of Sugarcane yellow leaf disease (ScYLD). High intensity of fluorescence in phloem region of infected compared to healthy plants was observed with aniline blue stain. The disease was successfully detected by Reverse transcription polymerase chain reaction (RT-PCR) with specific oligonucleotide primers. A distinct band at 320 bp was obtained from ScYLD infected plant samples but not from RNA extracted from healthy plant samples. Among 45 genotypes screened under natural conditions, 15 genotypes namely Co95005, CoC90063, CoVC93136, Co99006, Co99008, Co86249, Co95012, Co62175, Co03632, CoSnk.03754, Co85004, C094008, CoVC2003-165, Co0416 and CoSnk.3822 showed resistant reaction to the disease. While, 8 genotypes showed moderately resistant; 10 genotypes showed moderately susceptible and 12 genotypes showed susceptible reaction to the disease. The most popular cultivar Co86032 was found comparatively susceptible to the disease than other cultivars grown in the command area. The level of sucrose per cent, commercial cane sugar per cent and purity per cent in ScYLD infected canes was lower compared to the healthy canes. Physical methods viz., hot water treatment, hot air oven treatment and aerated steam therapy were not effective in eradicating the causal agent. | en_US |
| dc.identifier.other | Th-9430 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810022215 | |
| dc.language.iso | en | en_US |
| dc.pages | 81 | en_US |
| dc.publisher | University of Agricultural Sciences, Bangalore | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.subject | null | en_US |
| dc.theme | MOLECULAR DETECTION OF SUGARCANE YELLOW LEAF VIRUS | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | MOLECULAR DETECTION OF SUGARCANE YELLOW LEAF VIRUS AND FIELD REACTION OF SUGARCANE GENOTYPES FOR THEIR RESISTANCE | en_US |
| dc.type | Thesis | en_US |