MOLECULAR DETECTION OF SUGARCANE YELLOW LEAF VIRUS AND FIELD REACTION OF SUGARCANE GENOTYPES FOR THEIR RESISTANCE
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Date
2009-07-15
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University of Agricultural Sciences, Bangalore
Abstract
Survey conducted to assess the incidence of yellow leaf disease of
sugarcane in Cauvery command area of Southern Karnataka indicated the
occurrence of disease incidence up to 35.3 per cent. Among the three districts,
highest disease incidence of 35.3 per cent was in Mandya district and lowest
incidence was recorded in Chamarajnagar district (10.5 %). A fluorescent
microscopic technique using aniline blue dye was standardized for detection of
Sugarcane yellow leaf disease (ScYLD). High intensity of fluorescence in phloem
region of infected compared to healthy plants was observed with aniline blue stain.
The disease was successfully detected by Reverse transcription polymerase chain
reaction (RT-PCR) with specific oligonucleotide primers. A distinct band at 320 bp
was obtained from ScYLD infected plant samples but not from RNA extracted from
healthy plant samples.
Among 45 genotypes screened under natural conditions, 15 genotypes
namely Co95005, CoC90063, CoVC93136, Co99006, Co99008, Co86249,
Co95012, Co62175, Co03632, CoSnk.03754, Co85004, C094008, CoVC2003-165,
Co0416 and CoSnk.3822 showed resistant reaction to the disease. While, 8
genotypes showed moderately resistant; 10 genotypes showed moderately
susceptible and 12 genotypes showed susceptible reaction to the disease. The
most popular cultivar Co86032 was found comparatively susceptible to the
disease than other cultivars grown in the command area. The level of sucrose
per cent, commercial cane sugar per cent and purity per cent in ScYLD infected
canes was lower compared to the healthy canes. Physical methods viz., hot water
treatment, hot air oven treatment and aerated steam therapy were not effective in
eradicating the causal agent.
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